BackgroundBifunctional axonal guidance cue regulated by sulfated proteoglycans; attractive effects result from interactions with heparan sulfate proteoglycans (HSPGs), while the inhibitory effects depend on interactions with chondroitin sulfate proteoglycans (CSPGs). Ligand for receptor PLXNB3. In glioma cells, SEMA5A stimulation of PLXNB3 results in the disassembly of F-actin stress fibers, disruption of focal adhesions and cellular collapse as well as inhibition of cell migration and invasion through ARHGDIA-mediated inactivation of RAC1. May promote angiogenesis by increasing endothelial cell proliferation and migration and inhibiting apoptosis.
DescriptionSEMA5A Polyclonal Antibody. Unconjugated. Raised in: Rabbit.
Formulation0.03% Proclin 300, 50% Glycerol, 0.01M PBS, PH 7.4
SpecificityHuman
IsotypeIgG
Uniprot IDQ13591
PurificationProtein G purified
ImmunogenRecombinant Human Semaphorin-5A protein (201-400AA)
StorageShipped at 4°C. Upon delivery aliquot and store at -20°C (short-term) or -80°C (long-term). Avoid repeated freeze.
Alternative NamesSemaphorin-5A, Semaphorin-F, Sema F, SEMA5A, SEMAF
ApplicationELISA, IHC, IF; Recommended dilution: IHC:1:20-1:200, IF:1:50-1:500