Recombinant Transmembrane Protease Serine 2 Protein, Partial

Background
The protease TMPRSS2 plays an important role in the infection mechanism of human coronaviruses, such as SARS-CoV and SARS-CoV-2. Cell entry of human coronaviruses depends on the binding of the viral spike (S) glycoprotein to cellular ACE2 receptor and S protein priming by host cell protease TMPRSS2. This protein is commonly used to study cancer. Previous studies have shown that the encoding gene for TMPRSS2 protein was up-regulated by androgenic hormones in prostate cancer cells and down-regulated in androgen-independent prostate cancer tissue.

Description
The recombinant Transmembrane protease serine 2 (TMPRSS2) is produced by the expression of a target DNA sequence with 6xHis, N-terminal tag(s), in the yeast expression system. The target DNA sequence encodes the 106-492aa region of the TMPRSS2. This protein predicts a molecular weight of 44.8 kDa. Its purity is greater than 90% as determined by SDS-PAGE. And the bio-activity test of this protein is ongoing.

Purity
Greater than 90% as determined by SDS-PAGE.

Formulation
Lyophilized powder: The buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Species
Human

Calculated MW
44.8kDa

Expressed Host
Yeast (Expression Region 106-492aa)

Tag
N-terminal 6xHis-tagged

Uniprot ID
O15393

Storage
Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20deg;C to -80deg;C. Our default final concentration of glycerol is 50%.

Alternative Names
D16Ertd61e; Epitheliasin; FLJ41954; MGC6821; PP9284; PRSS10; Serine protease 10; TMPRSS2; TMPRSS2 ERG FUSION GENE, INCLUDED; TMPRSS2 ETV1 FUSION GENE, INCLUDED; TMPS2_HUMAN; Transmembrane protease serine 2 catalytic chain; Transmembrane protease, serine 2; Transmembrane protease, serine 2, EC 3.4.219