Description Recombinant SARS-CoV-2 Nucleocapsid Protein with His-Tag is produced by E. coli expression system. The target protein is expressed with sequence (Met1-Ala419) of SARS-COV-2 (2019-nCoV) Nucleocapsid (Accession #QHD43423.2) fused with a 6xHis tag at the N-terminus.
Bio-Activity Measured by its binding ability in a functional ELISA. Immobilized Recombinant SARS-COV-2 Nucleocapsid at 2ug/mL (100 uL/well) can bind Anti-Nucleocapsid antibody with a linear range of 0.12-1.64 ng/mL. Measured by its binding ability in a functional ELISA. Immobilized Recombinant SARS-CoV-2 envelope Protein at 2ug/mL (100 uL/well) can bind Recombinant SARS-CoV-2 Nucleocapsid protein, the EC50 of Recombinant SARS-CoV-2 Nucleocapsid protein is 41.10 ng/mL.
Purity > 95% by SDS-PAGE;> 95% by HPLC.
Endotoxin < 1.0 EU/ug of the protein by LAL method.
Formulation Lyophilized from a 0.22 um filtered solution of 20mM Tris, 500mM NaCl, 0.1mM EDTA, 10% glycerol, pH8.0 or Supplied as a 0.22 um filtered solution in 20mM Tris, 500mM NaCl, 0.1mM EDTA, 10% glycerol, pH8.0.
Species SARS-COV-2
Calculated MW 49.4kDa
Expressed Host E. coli
Tag 6xHis tag at the N-terminus Gene ID 43740575
Storage Store the lyophilized protein at -20°C to -80°C for long term. After reconstitution, the protein solution is stable at -20°C for 3 months, at 2-8°C for up to 1 week. or This product is stable at ≤ -70°C for up to 6 months from the date of receipt. For optimal storage, aliquot into smaller quantities after centrifugation and store at the recommended temperature. Reconstitution For lyophilized protein: Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water.
Alternative Names Nucleocapsid protein; NP; Nucleoprotein; cov np; Protein N; COVID-19; SARS-CoV-2; 2019-nCoV