NT5E Recombinant Monoclonal Antibody [6F6]

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IHC image of NT5E rMAb diluted at 1:100 and staining in paraffin-embedded human endometrial cancer performed on a Leica Bond™ system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6. 0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0. 05% DAB.

IHC image of NT5E rMAb diluted at 1:100 and staining in paraffin-embedded human colon cancer performed on a Leica Bond™ system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6. 0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0. 05% DAB.

Application:	ELISA, IHC
Areas of Research:	Nucleotide Metabolism
Clonality:	Monoclonal
Conjugate:	Unconjugated
Host:	HEK293F Cell
Isotype:	Rabbit IgG
Purification:	Affinity Chromatography
Reactivity:	Human
Recombinant:	Yes
100% Guarantee:	6 months

Catalog No.	Size	Price	Qty
A74352-050	50µl	$299.00
A74352-100	100µl	$399.00

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Product Overview
Datasheet & SDS
Product FAQ

Product Overview

Background
Hydrolyzes extracellular nucleotides into membrane permeable nucleosides. Exhibits AMP-, NAD-, and NMN-nucleosidase activities.

Description
NT5E Recombinant Monoclonal Antibody [6F6]. Unconjugated. Raised in: HEK293F Cell.

Formulation
Buffer: Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Specificity
Human

Isotype
Rabbit IgG

Uniprot ID
P21589

Purification
Affinity Chromatography

Immunogen
A synthesized peptide derived from human CD73

Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Alternative Names
5'-nucleotidase (5'-NT) (EC 3.1.3.5) (Ecto-5'-nucleotidase) (CD antigen CD73), NT5E, NT5 NTE

Application
ELISA, IHC; Recommended dilution: IHC:1:50-1:200

Datasheet & SDS

Datasheet/User Guide
If the above file is not available online, you can request a copy by contacting us.

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NT5E Recombinant Monoclonal Antibody [6F6]