Holiday Winter Sale Holiday Winter Sale: Use code E1812WTR to save 15% on your entire order. Ends 12/28/18. » Learn More
Epigentek Home
 
EpiGentek Guarantee

Histone H2AB/EK9ac (Acetyl H2AB/EK9) Monoclonal Antibody [RMC385A]

Share:

Write a Review
Immunocytochemistry analysis of Histone H2AB/EK9ac (Acetyl H2AB/EK9) Monoclonal Antibody [RMC385A] diluted at 1:100 and staining in Hela cells performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Immunofluorescence staining of Hela cells (treated by 15mM sodium butyrate for 30min) with Histone H2AB/EK9ac (Acetyl H2AB/EK9) Monoclonal Antibody [RMC385A] at 1:56, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).
Application: ELISA, ICC, IF
Areas of Research: Histone Acetylation & Deacetylation
Clonality: Monoclonal
Conjugate: Unconjugated
Host: Rabbit
Isotype: IgG
Purification: Affinity Purified
Reactivity: Human
100% Guarantee: 6 months
Catalog No.SizePriceQty
A68413-05050 µl $249.00 
A68413-100100 µl $399.00 
Availability: Usually Ships Within 1-2 Weeks 
Product Overview

Background
Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

Description
Histone H2AB/EK9ac (Acetyl H2AB/EK9) Monoclonal Antibody, clone RMC385A. Unconjugated. Raised in: Rabbit.

Concentration
Inquire

Formulation
Liquid. Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Specificity
Human

Isotype
IgG

Uniprot ID
P04908

Purification
Affinity Purified

Immunogen
A synthesized peptide

Storage
Shipped at 4°C. Upon receipt, store at -20°C (short-term) or -80°C (long-term). Avoid repeated freeze.

Alternative Names
Histone H2A type 1-B/E, Histone H2A.2, Histone H2A/a, Histone H2A/m, HIST1H2AB, H2AFM, AND, HIST1H2AE, H2AFA, Acetyl-Histone H2A type 1-B/E (K9)

Application
ELISA, ICC, IF; Recommended dilution: ICC: 1:50-1:500, IF: 1:30-1:200

VCU0006

User Guide & MSDS

[User Guide]*
*Always use the actual User Guide that shipped with your product. Is the above file locked? You can also request user guides by emailing info@epigentek.com along with your contact information and institution name.

Introduction to Epigenetics
Epigentek is an epigenetics company that specializes in epigenetic kits, antibodies, reagents, and services for epigenetic research in DNA methylation, histone modification, and chromatin studies. Terms & Conditions | Privacy Policy | Site Map
Copyright © 2017 Epigentek Group Inc. All rights reserved.