BackgroundAcetylation of the histone tail causes chromatin to adopt an "open" conformation, allowing increased accessibility of transcription factors to DNA. The identification of histone acetyltransferases (HATs) and their large mµltiprotein complexes has yielded important insights into how these enzymes regµlate transcription (1,2). HAT complexes interact with sequence-specific activator proteins to target specific genes. In addition to histones, HATs can acetylate nonhistone proteins, sµggesting mµltiple roles for these enzymes (3). In contrast, histone deacetylation promotes a "closed" chromatin conformation and typically leads to repression of gene activity (4). Mammalian histone deacetylases can be divided into three classes on the basis of their similarity to various yeast deacetylases (5). Class I proteins (HDACs 1, 2, 3, and 8) are related to the yeast Rpd3-like proteins, those in class II (HDACs 4, 5, 6, 7, 9, and 10) are related to yeast Hda1-like proteins, and class III proteins are related to the yeast protein Sir2. Inhibitors of HDAC activity are now being explored as potential therapeutic cancer agents (6,7).HDAC1 and HDAC2 are highly homologous and are involved in histone deacetylation, chromatin remodeling and transcriptional repression (8-10). Both proteins are found together in numerous complexes including the nucleosome remodeling and deacetylation complex (NuRD), MeCP1, and the mSin3A corepressor complex.
DescriptionHDAC2 Polyclonal Antibody. Unconjugated. Raised in: Rabbit.
FormulationLiquid. PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
SpecificityMouse, Rat
IsotypeIgG
Uniprot IDQ92769
PurificationAffinity Purified
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 429-488 of human HDAC2 (NP_001518.3).
StorageShipped at 4°C. Store at -20°C. Avoid multiple freeze/thaw cycles.
Alternative NamesHDAC2; HD2; RPD3; YAF1; histone deacetylase 2ApplicationWB, IHC; Recommended dilution: WB 1:500 - 1:2000, IHC 1:50 - 1:200
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