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FLII Polyclonal Antibody

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Immunofluorescence staining of Hela cells with FLII Polyclonal Antibody at 1:166, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).
Application: ELISA, IF, IHC
Areas of Research: Cell Development & Differentiation
Clonality: Polyclonal
Conjugate: Unconjugated
Host: Rabbit
Isotype: IgG
Purification: >95%, Protein G Purified
Reactivity: Human
Trial Size Available: Yes
100% Guarantee: 6 months
Catalog No.SizePriceQty
A68843-02020 µg $99.00 
A68843-05050 µg $249.00 
A68843-100100 µg $399.00 
Availability: Usually Ships Within 1-2 Weeks 
Product Overview

Background
May play a role as coactivator in transcriptional activation by hormone-activated nuclear receptors (NR) and acts in cooperation with NCOA2 and CARM1. Involved in estrogen hormone signaling. Involved in early embryonic development. May play a role in regulation of cytoskeletal rearrangements involved in cytokinesis and cell migration, by inhibiting Rac1-dependent paxillin phosphorylation.

Description
FLII Polyclonal Antibody. Unconjugated. Raised in: Rabbit.

Formulation
Liquid. 0.03% Proclin 300, 50% Glycerol, 0.01M PBS, pH 7.4.

Specificity
Human

Isotype
IgG

Uniprot ID
Q13045

Purification
>95%, Protein G purified

Immunogen
Recombinant Human Protein flightless-1 homolog protein (577-729AA)

Storage
Shipped at 4°C. Upon receipt, store at -20°C (short-term) or -80°C (long-term). Avoid repeated freeze.

Alternative Names
Protein flightless-1 homolog, FLII, FLIL

Application
ELISA, IHC, IF; Recommended dilution: IHC:1:500-1:1000, IF:1:50-1:200

User Guide & MSDS

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