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EpiQuik DNMT3B Activity/Inhibitor Screening Assay Core Kit

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For screening DNMT3B inhibitors in an ELISA-like format

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Schematic procedure for using the EpiQuik DNMT3B Activity/Inhibitor Screening Assay Core Kit.
Activity of recombinant DNMT3B at different concentrations was measured using the EpiQuik™ DNMT3B Activity/Inhibitor Screening Assay Core Kit.
Recombinant DNMT3B was incubated with substrate and inhibitor BD-11. DNMT3B activity was measured in the presence or absence of inhibitors.
Input Type: Purified Enzyme
Research Area: DNA Methylation
Target Application: Activity Measurement
Vessel Format: 96-Well Plate
100% Guarantee: 6 months
Catalog No.SizePriceQty
P-3007A-4848 assays $256.00 
P-3007A-9696 assays $483.00 
Availability: Usually Ships In 1-2 Days 
Product Overview

The EpiQuik™ DNMT3B Activity/Inhibitor Screening Assay Core Kit is a convenient set of tools that allows the experimenter to selectively screen DNMT3B activity/inhibitors in an ELISA-like format. The selective inhibition of DNMT3B may lead to demethylation and expression of silenced tumor suppressor genes. Thus, the selective DNMT3B inhibitors could be a new addition to cancer therapeutic agents. The kit is ready-to-use and provides all the essential components needed to carry out a successful DNMT3B activity/inhibitor screening experiment without the need for radioactivity or any special equipment. The kit has the following advantages:

  • Very rapid procedure, which can be completed within 3 hours.
  • Safe and innovative colorimetric assay without radioactivity, extraction, and chromatography.
  • Strip microplate format makes the assay flexible: manual or high throughput analysis.
  • Extremely simple, reliable, and consistent assay conditions.

Note: This "core" kit does not come with enzymes included. 

Principle & Procedure
The EpiQuik™ DNMT3B Activity/Inhibitor Screening Assay Core Kit is designed for screening Dnmt 3B inhibitors. In an assay with this kit, the unique cytosine-rich DNA substrate is stably coated on the strip wells. These wells are specifically treated to have a high DNA absorption ability. A DNMT3B (not included) enzyme transfers a methyl group to cytosine from Adomet to methylate DNA substrate. The methylated DNA can be recognized with an anti-5-methylcytosine antibody. The ratio or amount of methylated DNA, which is proportional to enzyme activity, can then be colorimetrically quantified through an ELISA-like reaction.

 
Fig. 1. Schematic procedure for using the EpiQuik DNMT3B Activity/Inhibitor Screening Assay Core Kit.

Fig. 2. Activity of recombinant DNMT3B at different concentrations was measured using the EpiQuik™ DNMT3B Activity/Inhibitor Screening Assay Core Kit.

Fig. 3. Recombinant DNMT3B was incubated with substrate and inhibitor BD-11. DNMT3B activity was measured in the presence or absence of inhibitors.

Product Components

MT1 (10X Wash Buffer)
MT2 (Dnmt Assay Buffer)
MT3 (Adomet)*
MT5 (Capture Antibody)*
MT6 (Detection Antibody)*
MT7 (Developing Solution)
MT8 (Stop Solution)
8-Well Substrate-Coated Strip (with Frame)
User Guide

* For maximum recovery of the products, centrifuge the original vial after thawing prior to opening the cap.

User Guide & MSDS

[User Guide]*
*Always use the actual User Guide that shipped with your product. Is the above file locked? You can also request user guides by emailing info@epigentek.com along with your contact information and institution name.

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