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Qu K et. al. (September 2023). Oscillatory shear stress-induced downregulation of TET1s injures vascular endothelial planar cell polarity by suppression of actin polymerization APL Bioeng. 7(3):036104.
In this study, researchers investigated the impact of oscillatory shear stress on vascular endothelial planar cell polarity and its potential role in atherosclerosis. They discovered that the downregulation of TET1s, a short isoform of Tet methylcytosine dioxygenase 1, under oscillatory shear stress led to the disruption of endothelial planar polarity by inhibiting F-actin polymerization. This mechanism involves the modulation of sFRP-1 expression by TET1s, which affects the interaction between sFRP-1 and Fzd4 and subsequently influences F-actin dynamics, providing new insights for preventing atherosclerosis development.
Products Used: 5-Methylcytosine (5-mC) Monoclonal Antibody [33D3], 5-Hydroxymethylcytosine (5-hmC) Monoclonal Antibody [HMC/4D9]
Zhao X et. al. (July 2023). Dot1l cooperates with Npm1 to repress endogenous retrovirus MERVL in embryonic stem cells Nucleic Acids Res.
The study investigates the role of Dot1l, a histone methyltransferase, in repressing endogenous retroviruses in embryonic stem cells (ESCs). The absence of Dot1l leads to the activation of MERVL, a marker of 2-cell-like cells, triggering differentiation toward the trophectoderm lineage, and the mechanism involves Dot1l's interaction with Npm1 and their collaboration in regulating protein levels and deposition of histone H1 to suppress MERVL.
Products Used: MuERVL-Gag Polyclonal Antibody
Su Z et. al. (July 2023). Hyperhomocysteinemia lowers serum testosterone concentration via impairing testosterone production in Leydig cells Cell Biol Toxicol.
The study reveals that hyperhomocysteinemia (HHcy) leads to lower serum testosterone levels in mice by impairing testosterone production in Leydig cells. The mechanism involves downregulation of key transcription factors for steroidogenic genes, such as Nr5a1, due to altered histone methylation, and the study also identifies a previously unknown cell cluster, Macrophage-like Leydig cells, which contribute to the inflammatory response in Leydig cells under HHcy conditions.
Products Used: EpiQuik Total Histone Extraction Kit
Okamura M et. al. (July 2023). Pharmacological inhibition of histone deacetylases ameliorates cognitive impairment after intracerebral hemorrhage with epigenetic alteration in the hippocampus J Stroke Cerebrovasc Dis. 32(9):107275.
The study explores the effects of exercise and histone deacetylase (HDAC) inhibition using sodium butyrate (NaB) on cognitive function and epigenetic factors in the hippocampus after intracerebral hemorrhage (ICH) in rats. The results indicate that repetitive NaB administration ameliorates cognitive impairment induced by ICH, suggesting the potential of HDAC inhibitors as a therapeutic approach for post-stroke cognitive impairment.
Products Used: EpiQuik Total Histone Extraction Kit
Baldini F et. al. (July 2023). Nuclear and chromatin rearrangement associate to epigenome and gene expression changes in a model of in vitro adipogenesis and hypertrophy Biochim Biophys Acta Mol Cell Biol Lipids. 1868(10):159368.
The study investigates nuclear and chromatin changes associated with adipocyte differentiation and hypertrophy in an in vitro model. The results reveal alterations in nuclear morphology, chromatin distribution, and epigenetic modifications during adipogenesis and hypertrophy, providing insights into the mechanisms underlying these processes and their potential implications for obesity treatment.
Products Used: MethylFlash Global DNA Methylation (5-mC) ELISA Easy Kit (Colorimetric)
Silva CS et. al. (July 2023). Gene expression analyses reveal potential mechanism of inorganic arsenic-induced apoptosis in zebrafish J Appl Toxicol.
The study examines the effects of sodium arsenite exposure on gene expression and apoptosis in zebrafish larvae. Through gene expression analyses, the study identifies alterations in signaling pathways related to apoptosis and suggests the involvement of the p53 pathway in sodium arsenite-induced apoptosis, while also noting changes in global DNA methylation, contributing to a better understanding of the mechanisms underlying arsenic toxicity.
Products Used: MethylFlash Global DNA Hydroxymethylation (5-hmC) ELISA Easy Kit (Colorimetric)
Wang KD et. al. (July 2023). Sanguinarine induces apoptosis in osteosarcoma by attenuating the binding of STAT3 to the SSBP1 promoter region Br J Pharmacol.
In this study, reasearchers investigate the potential of sanguinarine as an anticancer agent against osteosarcoma. It reveals that sanguinarine induces apoptosis in osteosarcoma cells by disrupting the JAK/STAT3 signaling pathway, relieving STAT3-mediated inhibition on SSBP1 transcription, and subsequently activating the downstream PI3K/Akt/mTOR signaling axis, providing insights into a novel therapeutic approach for osteosarcoma treatment.
Products Used: EpiQuik Chromatin Immunoprecipitation (ChIP) Kit
Li A et. al. (July 2023). GATA6 promotes fibrotic repair of tracheal injury through NLRP3 inflammasome-mediated epithelial pyroptosis Int Immunopharmacol. 123:110657.
The study investigates the role of GATA6 in tracheal injury repair and its impact on NLRP3 inflammasome-mediated epithelial pyroptosis. It finds that GATA6 promotes fibrotic repair in tracheal injury by upregulating NLRP3 inflammasome activation and epithelial pyroptosis, suggesting GATA6 as a potential therapeutic target for tracheal injury treatment.
Products Used: EpiQuik Chromatin Immunoprecipitation (ChIP) Kit
Jiang Y et. al. (July 2023). Downregulation of FTO in the hippocampus is associated with mental disorders induced by fear stress during pregnancy Behav Brain Res. :114598.
The article investigates the role of N6-methyladenosine (m6A) modification and the fat mass and obesity-associated protein (FTO) in mental disorders induced by fear stress during pregnancy. The findings reveal that downregulation of FTO in the hippocampus of pregnant rats with fear stress-induced mental disorders is associated with aberrant modifications and expressions of specific genes, which could contribute to mental disorders through PI3K/Akt and Rap1 signaling pathways.
Products Used: EpiQuik m6A RNA Methylation Quantification Kit (Colorimetric)
Li Z et. al. (July 2023). Effect of acute PM2.5 exposure on PTGS2 and RNA m6A modification Environ Pollut. 335:122264.
The study examines the impact of acute exposure to particulate matter 2.5 (PM2.5) on RNA m6A modification and PTGS2 gene expression. Through a panel study of human subjects, it is revealed that higher PM2.5 exposure is associated with increased global m6A RNA methylation and elevated PTGS2 expression in peripheral blood, suggesting a potential regulatory role of RNA m6A modification in the inflammatory response induced by PM2.5 exposure.
Products Used: EpiQuik m6A RNA Methylation Quantification Kit (Colorimetric)
