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Lu X et. al. (February 2024). High estrogen during ovarian stimulation induced loss of maternal imprinted methylation that is essential for placental development via overexpression of TET2 in mouse oocytes Cell Commun Signal. 22(1):135.
Researchers studied how high estrogen levels during ovarian stimulation in assisted reproductive technology affect DNA methylation in mouse oocytes. They found that too much estrogen reduced 5mC methylation and increased 5hmC methylation in oocytes and embryonic stem cells. This abnormal methylation pattern affected a gene important for placental development, suggesting risks for assisted reproduction procedures.
Products Used: MethylFlash Global DNA Methylation (5-mC) ELISA Easy Kit (Colorimetric), 5-Methylcytosine (5-mC) Monoclonal Antibody [33D3], 5-Hydroxymethylcytosine (5-hmC) Monoclonal Antibody [HMC/4D9], EpiQuik m6A RNA Methylation Quantification Kit (Colorimetric)
Zhu M et. al. (February 2024). N6-methyladenosine modification of the mRNA for a key gene in purine nucleotide metabolism regulates virus proliferation in an insect vector Zhu M et. al. (February 2024).
Researchers discovered that in insects infected with rice stripe virus (RSV), a key gene involved in nucleotide metabolism, LsIMPDH, is regulated by chemical modification N6-methyladenosine (m6A) on its mRNA. This modification reduces the enzyme's activity, limiting the production of a necessary nucleotide, which in turn limits the virus's ability to replicate in its insect vector. This finding suggests a potential target for controlling viral transmission in insects.
Products Used: EpiQuik m6A RNA Methylation Quantification Kit (Colorimetric), EpiQuik CUT&RUN m6A RNA Enrichment (MeRIP) Kit
Jing Y et. al. (February 2024). TaLBD41 interacts with TaNAC2 to regulate nitrogen uptake and metabolism in response to nitrate availability New Phytol.
This study investigates how the wheat transcription factor TaLBD41 interacts with TaNAC2 in response to nitrate availability, regulating nitrogen uptake and metabolism. The interaction between these two transcription factors coordinates nitrate uptake, reduction, and assimilation, influencing wheat adaptation to different nitrogen supply conditions.
Products Used: EpiQuik Chromatin Immunoprecipitation (ChIP) Kit
Liang Y et. al. (February 2024). FOSL1 regulates hyperproliferation and NLRP3-mediated inflammation of psoriatic keratinocytes through the NF-kB signaling via transcriptionally activating TRAF3 Biochim Biophys Acta Mol Cell Res. :119689.
The article explores the role of FOSL1, a transcription factor, in regulating hyperproliferation and inflammation of psoriatic keratinocytes through the NF-kB signaling pathway. Psoriasis, a common skin disease, involves dysregulated FOSL1 expression, which promotes cell proliferation and NLRP3-mediated inflammation by activating TRAF3. The findings suggest FOSL1 as a potential therapeutic target for managing psoriasis.
Products Used: EpiQuik Chromatin Immunoprecipitation (ChIP) Kit
Wang J et. al. (February 2024). TET1 inhibits liver fibrosis by blocking hepatic stellate cell activation J Gastroenterol Hepatol.
The article investigates how TET1, a hydroxymethylase enzyme, inhibits liver fibrosis by blocking the activation of hepatic stellate cells (HSCs), which are crucial in liver fibrosis development. TET1 levels were found to be reduced in liver fibrosis models and activated HSCs, and its overexpression inhibited HSC activation by regulating the transcription factor KLF2. These findings suggest TET1 as a potential therapeutic target for treating liver fibrosis.
Products Used: EpiQuik Hydroxymethylated DNA Immunoprecipitation (hMeDIP) Kit
Yang Z et. al. (February 2024). Histone deacetylase OsHDA706 orchestrates rice broad-spectrum antiviral immunity and is impeded by a viral effector Cell Rep. 43(3):113838.
The study investiagtes how the histone deacetylase OsHDA706 regulates rice's broad-spectrum antiviral immunity by stabilizing the enzyme OsLOX14, which is involved in jasmonic acid (JA) biosynthesis. OsHDA706 deacetylates OsLOX14, leading to JA accumulation and improved antiviral defense, but this process is hindered by the viral protein P2, promoting viral infection.
Products Used: EpiQuik HDAC Activity/Inhibition Assay Kit (Colorimetric)
Wang J et. al. (February 2024). The m6A reader IGF2BP1 attenuates the stability of RPL36 and cell proliferation to mediate benzene hematotoxicity by recognizing m6A modification Toxicology. 503:153758.
The article explores how the m6A reader protein IGF2BP1 is involved in benzene-induced hematotoxicity by recognizing m6A modifications. IGF2BP1 downregulation is observed in benzene-exposed workers and cells, and its overexpression alleviates benzene-induced hematopoietic damage in mice. Additionally, IGF2BP1 recognizes m6A modification on the Ribosomal Protein L36 (RPL36) mRNA, leading to reduced RPL36 stability, impaired cell proliferation, and ultimately contributing to benzene hematotoxicity.
Products Used: EpiQuik m6A RNA Methylation Quantification Kit (Colorimetric)
Li Z et. al. (February 2024). RNA m6A modification regulates L1 retrotransposons in human spermatogonial stem cell differentiation in vitro and in vivo Cell Mol Life Sci. 81(1):92.
The study investigates the role of N6-methyl-adenosine (m6A) modification in regulating L1 retrotransposons and human germline development. Knocking out the m6A methyltransferase METTL3 or the m6A reader YTHDF2 in human embryonic stem cells (hESCs) increased L1 retrotransposition and reduced the efficiency of spermatogonial stem cell (SSC) differentiation, highlighting the importance of m6A modification in safeguarding genome stability during germline development.
Products Used: EpiQuik m6A RNA Methylation Quantification Kit (Colorimetric)
Zhang S et. al. (February 2024). The m6A methylation and expression profiles of mouse neural stem cells after hypoxia/reoxygenation Stem Cell Res Ther. 15(1):43.
The study investigated the effects of hypoxia/reoxygenation (H/R) on mouse neural stem cells (NSCs) and their N6-methyladenosine (m6A) methylation profiles. They found that m6A modification levels and the expression of the m6A writer Mettl14 increased in NSCs after H/R. Through sequencing analysis, they identified key genes involved in NSC proliferation and migration, highlighting the role of m6A methylation in regulating these processes after H/R.
Products Used: EpiQuik m6A RNA Methylation Quantification Kit (Colorimetric)
Yu Y et. al. (February 2024). LRPPRC promotes glycolysis by stabilising LDHA mRNA and its knockdown plus glutamine inhibitor induces synthetic lethality via m6 A modification in triple-negative breast cancer Yu Y et. al. (February 2024).
The study explores how leucine-rich pentatricopeptide repeat-containing protein (LRPPRC) affects triple-negative breast cancer (TNBC) by regulating glycolysis through stabilizing LDHA mRNA. LRPPRC promotes TNBC progression by regulating metabolic reprogramming via m6A modification, highlighting potential combination targeted therapy strategies for TNBC.
Products Used: EpiQuik m6A RNA Methylation Quantification Kit (Colorimetric)
Yang G et. al. (February 2024). Combined GWAS and eGWAS reveals the genetic basis underlying drought tolerance in emmer wheat (Triticum turgidum L.) New Phytol.
The article investigates the genetic basis of drought tolerance in emmer wheat, a valuable genetic resource for breeding drought-tolerant wheat. Through a combined analysis of genome-wide association studies (GWAS) and expression quantitative trait loci (eQTLs), the research identifies numerous genetic loci and regulatory networks associated with drought tolerance in emmer wheat, offering valuable insights for improving drought tolerance in wheat breeding programs.
Products Used: EpiQuik CUT&RUN m6A RNA Enrichment (MeRIP) Kit
Liao M et. al. (February 2024). METTL3-mediated m6A modification of NORAD inhibits the ferroptosis of vascular smooth muscle cells to attenuate the aortic dissection progression in an YTHDF2-dependent manner Mol Cell Biochem.
The study investigates how the long non-coding RNA NORAD affects the ferroptosis (iron-dependent cell death) of vascular smooth muscle cells (VSMCs) and the progression of aortic dissection (AD). It is found that NORAD, regulated by the m6A methyltransferase METTL3 and recognized by the m6A reader YTHDF2, inhibits ferroptosis in VSMCs by stabilizing the GPX4 mRNA, which encodes a key ferroptosis inhibitor. This mechanism suggests that NORAD could be a potential therapeutic target for AD.
Products Used: EpiQuik CUT&RUN m6A RNA Enrichment (MeRIP) Kit
Scelfo A et. al. (April 2024). Tunable DNMT1 degradation reveals DNMT1/DNMT3B synergy in DNA methylation and genome organization J Cell Biol. 223(4)
The study explores DNA methylation (DNAme), a critical process for genome stability, using new cell models that allow inducible and reversible DNAme modulation through DNMT1 depletion. These models reveal a cooperative activity between DNMT1 and DNMT3B in regulating DNAme and genome organization, shedding light on the etiology of DNAme-related diseases.
Products Used: EpiQuik CUT&RUN m6A RNA Enrichment (MeRIP) Kit
