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Alghamdi AA et. al. (January 2024). Alterations in Histone Methylation States Increased Profusion of Lethal(2)-Essential-for-Life-Like (l(2)elf), Trithorax and Polycomb Genes in Apis mellifera under Heat Stress Insects. 15(1)
This study investigates histone methylation states in the honeybee Apis mellifera, specifically focusing on various histone modifications associated with key genes under heat stress. The results demonstrate significant changes in methylation/demethylation patterns, leading to increased expression of survival-associated genes (l(2)elf, trithorax) and decreased expression of genes related to general cellular activities (Polycomb).
Products Used: ChromaFlash Chromatin Extraction Kit, Histone H3K4me2 (H3K4 Dimethyl) Polyclonal Antibody, Histone H3K4me3 (H3K4 Trimethyl) Polyclonal Antibody, Histone H3K27me2 (H3K27 Dimethyl) Polyclonal Antibody, Histone H3K27me3 (H3K27 Trimethyl) Polyclonal Antibody
Dong C-L et. al. (January 2024). Exogenous methionine contributes to reversing the resistance of Streptococcus suis to macrolides Microbiol Spectr. :e0280323.
This study explores the potential of L-methionine in enhancing the effectiveness of macrolide antibiotics against Streptococcus suis, including multidrug-resistant strains. The research delves into the underlying mechanisms, highlighting the impact of exogenous methionine on methylation levels, efflux pump genes, oxidative stress, and metal starvation, offering insights for the rational use of macrolides and identifying a potential target for combating drug resistance in Streptococcus suis.
Products Used: MethylFlash Global DNA Methylation (5-mC) ELISA Easy Kit (Colorimetric)
Wydorski PJ et. al. (January 2024). Exposure to the extremely low-frequency electromagnetic field induces changes in the epigenetic regulation of gene expression in the endometrium Theriogenology. 217:72-82.
This article investigates the impact of extremely low-frequency electromagnetic fields (ELF-EMF), a recognized environmental risk factor, on the epigenetic regulation of gene expression in the porcine endometrium during the peri-implantation period. Exposure to ELF-EMF was found to induce changes in elements of epigenetic regulation, including alterations in DNA methylation, histone modification, and microRNA biogenesis, potentially disrupting biological processes in the uterus during peri-implantation.
Products Used: MethylFlash Methylated DNA 5-mC Quantification Kit (Colorimetric)
Jbeli AH et. al. (January 2024). Brg1/PRMT5 Nuclear Complex Epigenetically Regulates FOXO1 In IPF Mesenchymal Progenitor Cells Am J Physiol Lung Cell Mol Physiol.
In this study, intrinsically fibrogenic mesenchymal progenitor cells (MPCs) in human idiopathic pulmonary fibrosis (IPF) lungs were discovered and found to exhibit a distinct transcriptome, suggesting epigenetic modifications. The research reveals a Brg1/PRMT5 nuclear complex that epigenetically regulates the expression of the FOXO1 transcription factor in IPF MPCs, providing insights into the mechanisms driving IPF progression.
Products Used: Histone H3R2 Dimethyl Symmetric (H3R2me2s) Polyclonal Antibody
Zhang J et. al. (January 2024). FTO protein regulates the TGF-β signalling pathway through RNA N6-methyladenosine modification to induce unexplained recurrent spontaneous abortion FEBS J.
This article explores the role of RNA N6-methyladenosine (m6A) modification in unexplained recurrent spontaneous abortion (URSA) and reveals that the m6A demethylase FTO protein inhibits MEG3 m6A modification, weakening the stabilizing effect of YTHDC1 on MEG3. The study further uncovers the mechanism by which MEG3, influenced by FTO, regulates the TGF-β signaling pathway, impacting trophoblast invasion and proliferation in URSA trophoblast cells.
Products Used: EpiQuik m6A RNA Methylation Quantification Kit (Colorimetric)
Shen J et. al. (January 2024). Genome-wide screening of m6A profiling of cutaneous wound healing in diabetic mice Mol Biol Rep. 51(1):175.
This study systematically profiles the m6A modification landscape in cutaneous wounds of diabetic mice, revealing alterations in the expression of m6A-related enzymes and identifying differentially expressed m6A peaks. The findings provide new insights into the molecular mechanisms of diabetic wound healing, suggesting potential targets for future treatments.
Products Used: EpiQuik m6A RNA Methylation Quantification Kit (Colorimetric)
Chen H et. al. (January 2024). BMP9-ID1 Pathway Attenuates N6-Methyladenosine Levels of CyclinD1 to Promote Cell Proliferation in Hepatocellular Carcinoma Int J Mol Sci. 25(2)
The article investigates the role of the BMP9 signaling pathway in hepatocellular carcinoma (HCC) and its impact on N6-methyladenosine (m6A) methylation and cell cycle progression. Elevated BMP9 levels in HCC are associated with a poor prognosis, and the study demonstrates that BMP9 reduces global RNA m6A methylation, promoting cell cycle progression in HCC cells. The BMP9-ID1 pathway is identified as a key regulator, downregulating m6A methylation in CyclinD1 mRNA, suggesting its potential as a therapeutic target for HCC treatment.
Products Used: EpiQuik CUT&RUN m6A RNA Enrichment (MeRIP) Kit
Ferguson DT et. al. (January 2024). The Garlic Compound, Diallyl Trisulfide, Attenuates Benzo[a]Pyrene-Induced Precancerous Effect through Its Antioxidant Effect, AhR Inhibition, and Increased DNA Repair in Human Breast Epithelial Cells Nutrients. 16(2)
The article investigates the potential chemopreventive effects of diallyl trisulfide (DATS), a garlic organosulfur compound, against benzo[a]pyrene (B[a]P)-induced precancerous effects in human breast epithelial cells. DATS demonstrated antioxidant effects, inhibited the aryl hydrocarbon receptor (AhR), and increased DNA repair, attenuating B[a]P-induced changes associated with carcinogenesis in the study.
Products Used: EpiQuik 8-OHdG DNA Damage Quantification Direct Kit (Colorimetric)
