BackgroundCholine-specific glycerophosphodiester phosphodiesterase. The preferred substrate may be lysosphingomyelin. Hydrolyzes lysophosphatidylcholine (LPC) to form monoacylglycerol and phosphorylcholine but not lysophosphatidic acid, showing it has a lysophospholipase C activity. Has a preference for LPC with short (12:0 and 14:0) or polyunsaturated (18:2 and 20:4) fatty acids. Also hydrolyzes glycerophosphorylcholine and sphingosylphosphorylcholine efficiently. Hydrolyzes the classical substrate for phospholipase C, p-nitrophenyl phosphorylcholine in vitro, while it does not hydrolyze the classical nucleotide phosphodiesterase substrate, p-nitrophenyl thymidine 5'-monophosphate. Does not hydrolyze diacyl phospholipids such as phosphatidylethanolamine, phosphatidylinositol, phosphatidylserine, phosphatidylglycerol and phosphatidic acid.
DescriptionENPP6 Polyclonal Antibody. Unconjugated. Raised in: Rabbit.
FormulationLiquid. 0.03% Proclin 300, 50% Glycerol, 0.01M PBS, PH 7.4.
SpecificityHuman
IsotypeIgG
Uniprot IDQ6UWR7
Purification>95%, Protein G purified
ImmunogenRecombinant Human Ectonucleotide pyrophosphatase/phosphodiesterase family member 6 protein (31-330AA)
StorageShipped at 4°C. Upon delivery aliquot and store at -20°C (short-term) or -80°C (long-term). Avoid repeated freeze.
Alternative NamesEctonucleotide pyrophosphatase/phosphodiesterase family member 6, E-NPP 6, NPP-6, Choline-specific glycerophosphodiester phosphodiesterase, Glycerophosphocholine cholinephosphodiesterase, GPC-Cpde, ENPP6, UNQ1889/PRO4334
ApplicationELISA, IHC, IF; Recommended dilution: IHC:1:20-1:200, IF:1:50-1:200