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DAD1 Polyclonal Antibody

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All lanes: DAD1 Polyclonal Antibody at 2ug/ml
Lane 1: EC109 whole cell lysate
Lane 2: 293T whole cell lysate
Secondary
Goat polyclonal to Rabbit IgG at 1/15000 dilution
Predicted band size: 12 .4kDa
Observed band size: 12.4kDa
Immunohistochemistry of paraffin-embedded human prostate at dilution of 1:100
Immunofluorescent analysis of HepG2 cells at a dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)
Application: ELISA, IF, IHC, WB
Areas of Research: Apoptosis & Cell Death, Cell Metabolism
Clonality: Polyclonal
Conjugate: Unconjugated
Host: Rabbit
Isotype: IgG
Purification: Protein G Purified
Reactivity: Human
Trial Size Available: Yes
100% Guarantee: 6 months
Catalog No.SizePriceQty
A51601-02020 µg $99.00 
A51601-05050 µg $256.00 
A51601-100100 µg $359.00 
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Product Overview

Background
Component of the N-oligosaccharyl transferase enzyme which catalyzes the transfer of a high mannose oligosaccharide from a lipid-linked oligosaccharide donor to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains. N-glycosylation occurs cotranslationally and the complex associates with the Sec61 complex at the channel-forming translocon complex that mediates protein translocation across the endoplasmic reticulum (ER). Loss of the DAD1 protein triggers apoptosis.

Description
DAD1 Polyclonal Antibody. Unconjugated. Raised in: Rabbit.

Formulation
Liquid. 0.03% Proclin 300, 50% Glycerol, 0.01M PBS, PH 7.4.

Specificity
Human

Isotype
IgG

Uniprot ID
P61803

Purification
>95%, Protein G purified

Immunogen
Recombinant Human Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit DAD1 protein (1-113AA)

Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C (short-term) or -80°C (long-term). Avoid repeated freeze.

Alternative Names
Defender against cell death 1

Application
ELISA, WB, IHC, IF; Recommended dilution: WB:1:500-2000, IHC:1:20-1:200, IF:1:50-1:200

User Guide & MSDS

[User Guide]*
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