BackgroundNon-enzymatic component of the C3 and C5 convertases and thus essential for the propagation of the classical complement pathway. Covalently binds to immunoglobulins and immune complexes and enhances the solubilization of immune aggregates and the clearance of IC through CR1 on erythrocytes. C4A isotype is responsible for effective binding to form amide bonds with immune aggregates or protein antigens, while C4B isotype catalyzes the transacylation of the thioester carbonyl group to form ester bonds with carbohydrate antigens. Ref. 16 Ref. 17 Derived from proteolytic degradation of complement C4, C4a anaphylatoxin is a mediator of local inflammatory process. It induces the contraction of smooth muscle, increases vascular permeability and causes histamine release from mast cells and basophilic leukocytes.
DescriptionC4B Polyclonal Antibody, Biotin Conjugated. Biotin. Raised in: Rabbit.
FormulationLiquid. 0.03% Proclin 300, 50% Glycerol, 0.01M PBS, PH 7.4.
SpecificityHuman
IsotypeIgG
Uniprot IDP0C0L5
Purification>95%, Protein G purified
ImmunogenRecombinant Human Complement C4-B protein (1454-1744AA)
StorageShipped at 4°C. Upon delivery aliquot and store at -20°C (short-term) or -80°C (long-term). Avoid repeated freeze.
Alternative NamesBasic complement C4, C3 and PZP-like alpha-2-macroglobulin domain-containing protein 3, CO4, CPAMD3, C4B, C4B_2
ApplicationELISA; Recommended dilution: ELISA 1:500-1:1000