BAX Recombinant Monoclonal Antibody [15D9]

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Overlay Peak curve showing Hela cells stained with BAX rMAb (red line) at 1:50. The cells were fixed in 4% formaldehyde and permeated by 0. 2% TritonX-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1µg/1*10⁶cells) for 45min at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG(H+L) at 1:200 dilution for 35min at 4°C. Control antibody (green line) was rabbit IgG (1µg/1*10⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.

IHC image of BAX rMAb diluted at 1:50 and staining in paraffin-embedded mouse lung tissue performed on a Leica Bond™ system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6. 0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0. 53% DAB.

IHC image of BAX rMAb diluted at 1:50 and staining in paraffin-embedded human rectal cancer performed on a Leica Bond™ system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6. 0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0. 53% DAB.

Application:	ELISA, FC, IHC
Areas of Research:	Apoptosis & Cell Death
Clonality:	Monoclonal
Conjugate:	Unconjugated
Host:	HEK293F Cell
Isotype:	Rabbit IgG
Purification:	Affinity Chromatography
Reactivity:	Human
Recombinant:	Yes
100% Guarantee:	6 months

Catalog No.	Size	Price	Qty
A74016-050	50µl	$299.00
A74016-100	100µl	$399.00

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Product Overview
Datasheet & SDS
Product FAQ

Product Overview

Background
Plays a role in the mitochondrial apoptotic process. Under normal conditions, BAX is largely cytosolic via constant retrotranslocation from mitochondria to the cytosol mediated by BCL2L1/Bcl-xL, which avoids accumulation of toxic BAX levels at the mitochondrial outer membrane (MOM). Under stress conditions, undergoes a conformation change that causes translocation to the mitochondrion membrane, leading to the release of cytochrome c that then triggers apoptosis. Promotes activation of CASP3, and thereby apoptosis.

Description
BAX Recombinant Monoclonal Antibody [15D9]. Unconjugated. Raised in: HEK293F Cell.

Formulation
Buffer: Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Specificity
Human

Isotype
Rabbit IgG

Uniprot ID
Q07812

Purification
Affinity Chromatography

Immunogen
A synthesized peptide derived from Human BAX

Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Alternative Names
Apoptosis regulator BAX (Bcl-2-like protein 4) (Bcl2-L-4), BAX, BCL2L4

Application
ELISA, IHC, FC; Recommended dilution: IHC:1:50-1:200, FC:1:50-1:200

Datasheet & SDS

Datasheet/User Guide
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BAX Recombinant Monoclonal Antibody [15D9]