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ARSE Polyclonal Antibody

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IHC image of ARSE Polyclonal Antibody diluted at 1:400 and staining in paraffin-embedded human liver tissue performed on a Leica Bond™ system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
IHC image of ARSE Polyclonal Antibody diluted at 1:400 and staining in paraffin-embedded human pancreatic tissue performed on a Leica Bond™ system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Immunofluorescence staining of HepG2 cells with ARSE Polyclonal Antibody at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).
Application: ELISA, IF, IHC
Areas of Research: Cell Development & Differentiation
Clonality: Polyclonal
Conjugate: Unconjugated
Host: Rabbit
Isotype: IgG
Purification: >95%, Protein G Purified
Reactivity: Human
Trial Size Available: Yes
100% Guarantee: 6 months
Catalog No.SizePriceQty
A69399-02020 µg $59.00 
A69399-05050 µg $249.00 
A69399-100100 µg $399.00 
Availability: Usually Ships Within 1-2 Weeks 
Product Overview

Background
May be essential for the correct composition of cartilage and bone matrix during development. Has no activity toward steroid sulfates.

Description
ARSE Polyclonal Antibody. Unconjugated. Raised in: Rabbit.

Formulation
Liquid. 0.03% Proclin 300, 50% Glycerol, 0.01M PBS, pH 7.4.

Specificity
Human

Isotype
IgG

Uniprot ID
P51690

Purification
>95%, Protein G purified

Immunogen
Recombinant Human Arylsulfatase E protein (352-494AA)

Storage
Shipped at 4°C. Upon receipt, store at -20°C (short-term) or -80°C (long-term). Avoid repeated freeze.

Alternative Names
Arylsulfatase E, ASE, ARSE

Application
ELISA, IHC, IF; Recommended dilution: IHC: 1:200-1:500, IF: 1:50-1:200

User Guide & MSDS

[User Guide]*
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