BackgroundInvolved in sperm-egg adhesion. Upon fertilization sperm must first penetrate a layer of cumulus cells that surrounds the egg before reaching the zona pellucida. The cumulus cells are embedded in a matrix containing hyaluronic acid which is formed prior to ovulation. This protein aids in penetrating the layer of cumulus cells by digesting hyaluronic acid.
DescriptionSPAM1 Polyclonal Antibody. Unconjugated. Raised in: Rabbit.
FormulationLiquid. 0.03% Proclin 300, 50% Glycerol, 0.01M PBS, PH 7.4.
SpecificityHuman
IsotypeIgG
Uniprot IDP38567
Purification>95%, Protein G purified
ImmunogenRecombinant Human Hyaluronidase PH-20 protein (36-490AA)
StorageShipped at 4°C. Upon delivery aliquot and store at -20°C (short-term) or -80°C (long-term). Avoid repeated freeze.
Alternative NamesHyal-PH20, Hyaluronoglucosaminidase PH-20, Sperm adhesion molecule 1, Sperm surface protein PH-20, HYAL3, PH20, SPAM1
ApplicationELISA; Recommended dilutions: ELISA 1:500 to 1:2000