BackgroundSARS-CoV-2 harbors a unique furin cleavage site at the boundary between the S1 and S2 subunits of its spike protein, which can be cleaved by the proprotein convertase (PC) furin and furin-like PCs. Proteolytic activation of SARS-CoV-2 spike protein at the S1/S2 boundary facilitates interaction with host ACE2 receptor for cell entry. The complete SARS-CoV-2 furin cleavage site has been characterized as a 20 amino acid motif corresponding to the amino acid sequence A672- S691 of SARS-CoV-2 spike protein, with one core region SPRRAR│SV (8 amino acids, S680- V687) and two flanking solvent-accessible regions (8 amino acids, A672– N679, and 4 amino acids, A688- S691). The core region is very unique as its R683 and A684 positions are positively-charged (Arg) and hydrophobic (Ala) residues, respectively, which could be cleaved by furin and/or furin-like PCs secreted from host cells and bacteria in the airway epithelium. Furin and furin-like PCs, such as PC5/6A and PACE4, are proven to be cleavage region sequence-specific, and these PCs exhibit widespread tissue distribution.
DescriptionSARS-CoV-2 Furin Site Blocking Polyclonal Antibody. A novel antibody against the furin cleavage site of SARS-CoV-2 spike protein. This antibody is suitable for SARS-CoV-2 Spike protein detection by ELISA and IP, SARS-CoV-2 furin site cleavage blockage, and SARS-CoV-2 spike protein neutralization. Unconjugated. Raised in: Rabbit.
FormulationLiquid. 50% glycerol, PBS, pH 7.4
Concentration0.5 mg/ml
SourceHuman
SpecificityS1/S2 furin cleavage site of SARS-CoV-2 spike protein
IsotypeIgG
Uniprot IDP0DTC2
PurificationAffinity Purified
ImmunogenPeptide-KLH conjugate consisting of the SARS-CoV-2-specific furin motif (20 aa)
StorageShipped at 4°C. Upon receipt, store at 4°C for short term (3-4 weeks) and -20°C for long term (18 months). Avoid repeated freeze.
Alternative NamesfbAB; anti-SARS-CoV-2 S protein, anti- SARS-CoV-2 Spike glycoproteinn, anti-nCoV-19, anti-COVID-19
ApplicationELISA, IP, SARS-CoV-2-specific furin cleavage site blockage; Recommended dilution: ELISA: 1:500-1:5000; IP: 2-10 µg (per reaction)