N Recombinant Monoclonal Antibody [4D2]

Background
This gene plays a pivotal role in viral assembly by packaging the positive-strand viral genome RNA into a helical ribonucleocapsid (RNP), interacting crucially with the viral genome and membrane protein M. It enhances the efficiency of subgenomic viral RNA transcription and viral replication. It may interrupt host chemokine functions in vivo, potentially aiding viral replication and transmission. This effect is achieved by secretion into the extracellular space, where it competes with host chemokines for binding to host glycosaminoglycans (GAG). It might trigger inflammasome responses in cultured cells and mice, interacting with host NLRP3 to facilitate inflammasome assembly. This assembly then induces cytokine release, potentially playing a role in COVID lung injury.

Description
N Recombinant Monoclonal Antibody [4D2]. Unconjugated. Raised in: HEK293F Cell.

Formulation
Buffer: 0.03% Proclin 300, 50% Glycerol, 0.01M PBS, PH 7.4.

Specificity
Human Novel Coronavirus (SARS-CoV-2/ 2019-nCoV)

Isotype
Mouse scFv fusion with human IgG1 Fc

Uniprot ID
P0DTC9

Purification
Affinity Chromatography

Immunogen
Recombinant Human Novel Coronavirus Nucleoprotein (N) (1-419aa)

Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Alternative Names
Nucleocapsid protein, NC, protein N, N

Application
ELISA, GICA; Recommended dilution: ELISA:1:10000-1:50000, GICA:1:500-1:5000