Brilliantly Investigate DNA or RNA Stress Damage

Oxidative stress damage plays an important role in the etiology and progression of a multitude of human pathologies, including oncologic, cardiovascular, and autoimmune diseases and disorders. Through reactive oxygen species (ROS), oxidative stress can result in cellular damage, enzyme inactivation, and epigenetic impairment of DNA. A better understanding of oxidative stress damage—disease relationships can therefore be beneficial for diagnostic and therapeutic purposes. As a consequence, various biomarkers have been identified for oxidative stress damage, with the most well-studied being: oxidized nucleoside derivatives like 8-hydroxy-2’-deoxyguanosine (8-OH-dG) and 8-hydroxyguanosine (8-OH-G) that are generated by ROS in cellular DNA and RNA, respectively; the ROS hydrogen peroxide (H₂0₂) and its catalyzing enzyme superoxide dismutase (SOD); and histone H2AX phosphorylation, particularly at serine 139, which manifests during the DNA damage response.

Most available methods used to investigate these biomarkers are time-consuming and have low throughput, low accuracy, and high costs. By leveraging its EpiQuik™ technologies, EpiGentek has developed a series of innovative research tools to address those issues. Our comprehensive product suite for oxidative stress damage assessment includes the following features and advantages:

• Colorimetric/fluorometric ELISA-based assays with easy-to-follow steps for convenience and speed
• Fast procedure
• High sensitivity and specificity
• Assay flexibility via strip-well microplate format for either manual or high throughput analysis
• Simple, reliable, and consistent assay conditions

Choose The Right Oxidative Stress Damage Kit For Your Study

Exhibit Oxidative
Stress Damage
at the DNA Level

Reveal Oxidative
Stress Damage
at the RNA Level

Display Oxidative
Stress Damage at the
Cell/Tissue Extracts Level

Show Oxidative
Stress Damage at the
In-situ Cell Level

DNA Damage Quantification Kits

Detect & Quantify Oxidative DNA Damage (8-OHdG) Directly from DNA

A few notable benefits include:

• Fast procedure can be completed within 3 hours and 45 minutes
• Detection limit can be as low as 1 pg of 8-OHdG
• High specificity by detecting only 8-OHdG without cross-reactivity to 8-OHdG analogues
  
• Highly correlated with and close to HPLC or LC-MS analysis
• Manual or high throughput capabilities

Catalog #	Name	Description
P-6003	EpiQuik 8-OHdG DNA Damage Quantification Direct Kit (Colorimetric)	Colorimetric-based quantitation of 8-OH-dG in DNA samples (LOD = 2 pg of 8-OH-dG)
P-6004	EpiQuik 8-OHdG DNA Damage Quantification Direct Kit (Fluorometric)	Fluorometric assay for quantitating 8-OH-dG in DNA samples (LOD = 1 pg of 8-OH-dG)

RNA Damage Quantification Kit

Directly Detect Oxidative RNA Damage (8-OhG) Status Using RNA

A few notable benefits include:

• Fast procedure can be completed within 3 hours
• Detection limit can be as low as 2 pg of 8-OhG
• High specificity by detecting only 8-OhG within the indicated concentration range of the sample RNA
  
• Uses RNA isolated from cells or tissues, no need for RNA digestion or hydrolysis
• Manual or high throughput capabilities

Catalog #	Name	Description
P-6008	EpiQuik 8-OhG RNA Damage Quantification Direct Kit (Colorimetric)	Colorimetric-based quantitation of 8-OH-G in RNA samples (LOD = 2 pg of 8-OH-G)

Cell/Tissue Extract Oxidative Stress Damage Assays

Fast & Sensitive DNA Damage Measurement from Various Cells or Tissues

A few notable benefits include:

• Fast, easy-to-follow ELISA-like kits
• High sensitivity for detection from small input amounts
• Measurement from various samples
• Manual or high throughput capabilities

Catalog #	Name	Description
P-6005	EpiQuik In-situ and Ex-Situ Hydrogen Peroxide (H2O2) Assay Kit	Fast and sensitive measurement of H2O2 from various samples, including living cells, cell/tissue extracts, and body fluids
OP-0001	EpiQuik Superoxide Dismutase Activity/Inhibition Assay Kit (Colorimetric)	Detects SOD activity in cell/tissue extracts by measuring color production of a reaction mediated by the SOD substrate superoxide anion

In-Situ Oxidative Stress Damage Kits

Detect DNA Damage In-Situ from Various Samples

A few notable benefits include:

• Fast, easy-to-follow ELISA-like kits
• High sensitivity for detection from small input amounts
• Measurement from various samples
• Manual or high throughput capabilities

Catalog #	Name	Description
P-6001	EpiQuik In Situ DNA Damage Assay Kit	Detects DNA damage or apoptosis by measuring phospho-H2AX (Ser139) in cultured adherent cells
P-6005	EpiQuik In-situ and Ex-Situ Hydrogen Peroxide (H2O2) Assay Kit	Fast and sensitive measurement of H2O2 from various samples, including living cells, cell/tissue extracts, and body fluids

To offer you a complete repertoire of tools to use durring your Oxidative Stress Damage studies, EpigenTek also provides the following for your convenience:

• Sample Preparation Kits for DNA Extraction, RNA Extraction, and Nuclear Extraction
• A variety of high-quality DNA Damage & Repair Antibodies