NSDHL Polyclonal Antibody

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Western Blot
Positive WB detected in: Rat heart tissue
All lanes: NSDHL antibody at 7.2ug/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 42 KDa
Observed band size: 42 KDa

IHC image of NSDHL Polyclonal Antibody diluted at 1:400 and staining in paraffin-embedded human liver tissue performed on a Leica Bond™ system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

IHC image of NSDHL Polyclonal Antibody diluted at 1:400 and staining in paraffin-embedded human cervical cancer performed on a Leica Bond™ system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Immunofluorescence staining of Hela cells with NSDHL Polyclonal Antibody at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).

Application:	ELISA, IF, IHC, WB
Areas of Research:	Cell Metabolism
Clonality:	Polyclonal
Conjugate:	Unconjugated
Host:	Rabbit
Isotype:	IgG
Purification:	Protein G Purified
Reactivity:	Human, Rat
Trial Size Available:	Yes
100% Guarantee:	6 months

Catalog No.	Size	Price	Qty
A69871-020	20 µl	$111.00
A69871-050	50 µl	$277.00
A69871-100	100 µl	$445.00

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Product Overview
Datasheet & SDS
Product FAQ

Product Overview

Background
Involved in the sequential removal of two C-4 methyl groups in post-squalene cholesterol biosynthesis.

Description
NSDHL Polyclonal Antibody. Unconjugated. Raised in: Rabbit.

Formulation
0.03% Proclin 300. 50% Glycerol, 0.01M PBS, pH 7.4.

Specificity
Human, Rat

Isotype
IgG

Uniprot ID
Q15738

Purification
Protein G purified

Immunogen
Recombinant Human Sterol-4-alpha-carboxylate 3-dehydrogenase, decarboxylating protein (154-211AA)

Storage
Shipped at 4°C. Upon receipt, store at -20°C (short-term) or -80°C (long-term). Avoid repeated freeze.

Alternative Names
Sterol-4-alpha-carboxylate 3-dehydrogenase, decarboxylating, Protein H105e3, NSDHL, H105E3

Application
ELISA, WB, IHC, IF; Recommended dilution: WB:1:500-1:5000, IHC:1:200-1:500, IF:1:50-1:200

Datasheet & SDS

Datasheet/User Guide
If the above file is not available online, you can request a copy by contacting us.

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NSDHL Polyclonal Antibody