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Histone H2BK Monoclonal Antibody [RMC411A]

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Western Blot
Positive WB detected in: Mouse brain tissue, Rat liver tissue, Rat lung tissue
All lanes: Histone H2BK Monoclonal Antibody [RMC411A] at 0.16 ug/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 14 KDa
Observed band size: 14 KDa
IHC image of Histone H2BK Monoclonal Antibody [RMC411A] diluted at 1:100 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Application: ELISA, IHC, WB
Areas of Research: Unmodified Histone
Clonality: Monoclonal
Conjugate: Unconjugated
Host: Rabbit
Isotype: IgG
Purification: Affinity Purified
Reactivity: Human
100% Guarantee: 6 months
Catalog No.SizePriceQty
A68437-05050 µl $249.00 
A68437-100100 µl $399.00 
Availability: Usually Ships Within 1-2 Weeks 
Product Overview

Background
Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

Description
Histone H2BK Monoclonal Antibody, clone RMC411A. Unconjugated. Raised in: Rabbit.

Concentration
Inquire

Formulation
Liquid. Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Specificity
Human

Isotype
IgG

Uniprot ID
O60814

Purification
Affinity Purified

Immunogen
A synthesized peptide

Storage
Shipped at 4°C. Upon receipt, store at -20°C (short-term) or -80°C (long-term). Avoid repeated freeze.

Alternative Names
Histone H2B type 1-K, H2B K, HIRA-interacting protein 1, HIST1H2BK, H2BFT, HIRIP1

Application
ELISA, WB, IHC; Recommended dilution: WB: 1:5000-1:10000, IHC: 1:50-1:500

VCU0006

User Guide & MSDS

[User Guide]*
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