Histone H2BBK20ac (Acetyl H2BBK20) Monoclonal Antibody [RMC402A]

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Western Blot
Positive WB detected in: Hela whole cell lysate treated by 15mM sodium butyrate for 30min
All lanes: Histone H2BBK20ac (Acetyl H2BBK20) Monoclonal Antibody [RMC402A] at 0.135 ug/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 15 KDa
Observed band size: 15 KDa

Immunocytochemistry analysis of Histone H2BBK20ac (Acetyl H2BBK20) Monoclonal Antibody [RMC402A] diluted at 1:100 and staining in Hela cells performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Immunofluorescence staining of Hela cells (treated by 15mM sodium butyrate for 30min) withHistone H2BBK20ac (Acetyl H2BBK20) Monoclonal Antibody [RMC402A] at 1:84, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).

Overlay histogram showing Hela cells stained with Histone H2BBK20ac (Acetyl H2BBK20) Monoclonal Antibody [RMC402A] (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then permeabilized with 0.3% Triton X-100 for 2 min. The cells were then incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4°C. The secondary antibody used was FITC goat anti-rabbit IgG (H+L) at 1/200 dilution for 1 h at 4°C. Control antibody (green line) was used under the same conditions. Acquisition of >10,000 events was performed.

Application:	ELISA, FC, ICC, IF, WB
Areas of Research:	Histone Acetylation & Deacetylation
Clonality:	Monoclonal
Conjugate:	Unconjugated
Host:	Rabbit
Isotype:	IgG
Purification:	Affinity Purified
Reactivity:	Human, Mouse
100% Guarantee:	6 months

Catalog No.	Size	Price	Qty
A68434-050	50 µl	Discontinued		Discontinued
A68434-100	100 µl	$445.00

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Product Overview
Datasheet & SDS
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Product Overview

Background
Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

Description
Histone H2BBK20ac (Acetyl H2BBK20) Monoclonal Antibody, clone RMC402A. Unconjugated. Raised in: Rabbit.

Concentration
Inquire

Formulation
Liquid. Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Specificity
Human, Mouse

Isotype
IgG

Uniprot ID
P33778

Purification
Affinity Purified

Immunogen
A synthesized peptide

Storage
Shipped at 4°C. Upon receipt, store at -20°C (short-term) or -80°C (long-term). Avoid repeated freeze.

Alternative Names
Histone H2B type 1-B, Histone H2B.1, Histone H2B.f, H2B/f, HIST1H2BB, H2BFF, Acetyl-Histone H2B type 1-B (K20)

Application
ELISA, WB, ICC, IF, FC; Recommended dilution: WB: 1:5000-1:10000, ICC: 1:50-1:500, IF: 1:30-1:200

VCU0006

Datasheet & SDS

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If the above file is not available online, you can request a copy by contacting us.

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Histone H2BBK20ac (Acetyl H2BBK20) Monoclonal Antibody [RMC402A]