Cart (0)
Wang S et. al. (April 2024). DNA methylation regulates the secondary metabolism of saponins to improve the adaptability of Eleutherococcus senticosus during drought stress BMC Genomics. 25(1):330.
The article investigates how DNA methylation regulates the secondary metabolism of saponins in Eleutherococcus senticosus (E. senticosus) during drought stress. Drought stress can alter DNA methylation, impacting gene expression and product synthesis in plants. The study found that moderate water deprivation reduced DNA methylation levels, particularly at the promoters of genes involved in saponin synthesis. This demethylation allowed transcription factors to bind to the promoters, promoting gene expression and saponin synthesis. The increased saponin levels acted as antioxidants, enhancing E. senticosus' adaptability to drought stress.
Products Used: MethylFlash Global DNA Methylation (5-mC) ELISA Easy Kit (Colorimetric)
Jiang X et. al. (March 2024). Decreased vitamin D bio-availability with altered DNA methylation of its metabolism genes in association with the metabolic disorders among the school-aged children with degree I, II and III obesity J Nutr Biochem. :109627.
The study examines the link between obesity and vitamin D (VD) metabolism in school-aged children. It includes 106 obese children, categorized by degree of obesity, compared to a control group. Results show that as obesity worsened, there were significant changes in body composition, clinical characteristics, and VD metabolism. Obese children had higher serum VD concentrations but lower levels of VD metabolites and related hydroxylases compared to the control group. DNA methylation patterns of VD metabolism genes were negatively correlated with obesity severity, suggesting that obesity may affect VD metabolism through abnormal DNA methylation. These findings highlight the impact of obesity on VD bioavailability and metabolism, independent of BMI.
Products Used: MethylFlash Global DNA Hydroxymethylation (5-hmC) ELISA Easy Kit (Colorimetric)
Gu W et. al. (March 2024). Particulate polycyclic aromatic hydrocarbons and metals, DNA methylation and DNA methyltransferase among middle-school students in China Sci Total Environ. 926:172087.
This study investigates how particulate matter (PM) components affect DNA methylation and DNA methyltransferases (DNMTs). High PM exposure was linked to lower global DNA methylation and DNMT3A expression. Urinary arsenic (As), serum BPDE-albumin adduct, and urinary cadmium (Cd) were negatively correlated with % 5mC and DNMT expression. Mixture exposure was also associated with decreased DNMT3A expression, suggesting PM components can impact DNA methylation and DNMTs in children.
Products Used: MethylFlash Methylated DNA Quantification Kit (Fluorometric)
Kim Y et. al. (March 2024). ELAVL2 loss promotes aggressive mesenchymal transition in glioblastoma NPJ Precis Oncol. 8(1):79.
This study focuses on the RNA-binding protein ELAVL2 and its role in regulating aggressive MES transformation in GBM. ELAVL2 is frequently deleted in GBM and associated with specific clinical and molecular features. Loss of ELAVL2 promotes MES processes and chemo-resistance, while its overexpression has the opposite effect. High ELAVL2 expression is linked to better survival in GBM patients. ELAVL2 directly binds to EMT-inhibitory molecules' transcripts, affecting their stability, possibly through an m6A-dependent mechanism. These findings highlight ELAVL2's role as a critical tumor suppressor and mRNA stabilizer in GBM, impacting transcriptomic plasticity and glioma progression.
Products Used: EpiQuik m6A RNA Methylation Quantification Kit (Colorimetric)
Chen J et. al. (March 2024). Classical swine fever virus non-structural protein 5B hijacks host METTL14-mediated m6A modification to counteract host antiviral immune response PLoS Pathog. 20(3):e1012130.
The article explores how Classical Swine Fever Virus (CSFV) utilizes m6A modifications to evade the host's immune response and promote its proliferation. The researchers found that CSFV infection increases m6A modifications, particularly by upregulating the methyltransferase METTL14. The virus's non-structural protein 5B prevents METTL14 degradation by hijacking the E3 ubiquitin ligase HRD1. Further analysis revealed that METTL14 targets and methylates TLR4, leading to its accelerated degradation and suppression of TLR4-mediated immune signaling. These findings offer insights into CSFV's evasion strategies and suggest targeting METTL14 as a potential antiviral approach.
Products Used: EpiQuik m6A RNA Methylation Quantification Kit (Colorimetric)
Jia D et. al. (March 2024). Abscisic acid activates transcription factor module MdABI5-MdMYBS1 during carotenoid-derived apple fruit coloration Plant Physiol.
This study investigates the role of m6A-modified circRNA388 in the innate immunity of the sea cucumber Apostichopus japonicus. Results show that m6A-modified circRNA388, mainly detected in the cytoplasm, promotes coelomocyte autophagy and reduces intracellular Vibrio splendidus levels by sponging miR-2008 and activating the AjULK-mediated autophagy pathway. The nuclear export of m6A-modified circRNA388 is facilitated by AjYTHDC1 and AjCRM1, highlighting their roles in regulating circRNA function in sea cucumber immunity.
Products Used: EpiQuik Plant ChIP Kit
Hoang NM et. al. (March 2024). Tunable DNMT1 degradation reveals DNMT1/DNMT3B synergy in DNA methylation and genome organization Cell Rep Med. :101484.
This study investigates tunable DNMT1 degradation to reveal DNMT1/DNMT3B synergy in DNA methylation and genome organization. The researchers developed cell models allowing inducible and reversible DNA methylation modulation through DNMT1 depletion. They found that DNMT1 and DNMT3B, but not DNMT3A, cooperate to maintain and control DNA methylation. Gradual DNA methylation loss led to reversible changes in heterochromatin, compartmentalization, and peripheral localization. The study highlights the system's utility for studying DNMTs and DNA methylation with fine temporal resolution, offering insights into DNA methylation dysfunction and human disease.
Products Used: 5-Methylcytosine (5-mC) Monoclonal Antibody [33D3]
Liu C et. al. (April 2024). STUB1 is acetylated by KAT5 and alleviates myocardial ischemia-reperfusion injury through LATS2-YAP-β-catenin axis Commun Biol. 7(1):396.
This article examines how KAT5 acetylation of STUB1 reduces myocardial ischemia-reperfusion injury (MIRI) by regulating the LATS2-YAP-β-catenin axis. They found that KAT5 and STUB1 were downregulated, while LATS2 was upregulated in MIRI models. KAT5 promoted STUB1 transcription via acetylation, leading to LATS2 degradation and YAP/β-catenin pathway activation. Targeting this axis could be a potential therapeutic strategy for MIRI.
Products Used: Histone H3K27ac (Acetyl H3K27) Polyclonal Antibody
Qian P et. al. (April 2024). Transcriptional Expression of Histone Acetyltransferases and Deacetylases During the Recovery of Acute Exercise in Mouse Hippocampus J Mol Neurosci. 74(2):34.
The study investigates the expression of histone acetyltransferases (HATs) and deacetylases (HDACs) during the recovery period after acute exercise in the mouse hippocampus. It analyzes gene expression patterns and protein acetylation levels following exercise. The findings suggest that acute exercise leads to dynamic changes in HATs, HDACs, and protein acylation in the hippocampus.
Products Used: EpiQuik Total Histone Extraction Kit
