Epigenetic Research Papers 4/10/23 - 4/14/23

Yin S et. al. (March 2023). CDK5-PRMT1-WDR24 signaling cascade promotes mTORC1 signaling and tumor growth. Cell Rep. 42(4):112316.
The paper investigates the role of CDK5-PRMT1-WDR24 signaling cascade in promoting mTORC1 signaling and tumor growth. The study identifies PRMT1 as a critical regulator of GATOR2 that activates mTORC1 pathway through CDK5 phosphorylation, translocation, and lysosomal methylation of WDR24. Disrupting this axis suppresses HCC cell proliferation and xenograft tumor growth, suggesting a potential target for cancer therapy.
(Products Used: Histone H4R3 Dimethyl Asymmetric (H4R3me2a) Polyclonal Antibody, Histone H4R3 Dimethyl Symmetric (H4R3me2s) Polyclonal Antibody, EpiQuik Total Histone Extraction Kit)

Liang A et. al. (March 2023). m6A reader IGF2BP1 accelerates apoptosis of high glucose-induced vascular endothelial cells in a m6A-HMGB1 dependent manner Cell Rep. 42(4):112316.
The paper investigates the role of m6A reader IGF2BP1 in regulating apoptosis of high glucose-induced vascular endothelial cells in a m6A-HMGB1 dependent manner. The study found that IGF2BP1 was up-regulated in HG-treated HUVECs, and its knockdown recovered cell proliferation inhibited by HG-administration, reduced apoptosis, and stabilized the expression of m6A-modified RNA HMGB1. Therefore, the study suggests that IGF2BP1 serves as a target for developing diabetic angiopathy therapeutics.
(Products Used: EpiQuik m6A RNA Methylation Quantification Kit (Colorimetric))

Lin C et. al. (March 2023).METTL3 enhances pancreatic ductal adenocarcinoma progression and gemcitabine resistance through modifying DDX23 mRNA N6 adenosine methylation Cell Death Dis. 14(3):221.
The paper investigates the role of METTL3 in modifying DDX23 mRNA N6 adenosine methylation and enhancing pancreatic ductal adenocarcinoma (PDAC) progression and gemcitabine resistance. The study found that METTL3 was upregulated in PDAC tissues with gemcitabine resistance, and its knockdown sensitized pancreatic cancer cells to chemotherapy, reduced cell proliferation, migration, and invasion both in vitro and in vivo. The study suggests that METTL3/DDX23 axis has a potential tumor promotive and chemo-resistant role in PDAC.
(Products Used: EpiQuik m6A RNA Methylation Quantification Kit (Colorimetric))

Tian S et. al. (March 2023). Chinese Ecliptae herba (Eclipta prostrata (L.) L.) extract and its component wedelolactone enhances osteoblastogenesis of bone marrow mesenchymal stem cells via targeting METTL3-mediated m6A RNA methylation J Ethnopharmacol. :116433.
The research investigated the mechanism through which Ecliptae herba and its component wedelolactone promote osteoblast differentiation from bone marrow mesenchymal stem cells (BMSC). The study found that the increased expression of methyltransferase METTL3 and METTL14 by Ecliptae herba led to an increase in mRNA m6A modification, which contributed to the enhancement of osteoblastogenesis. The findings suggest a potential role for Ecliptae herba and wedelolactone in treating osteoporosis by targeting METTL3-mediated m6A RNA methylation.
(Products Used: EpiQuik m6A RNA Methylation Quantification Kit (Colorimetric))

Liu J et. al. (April 2023). m6A methyltransferase METTL3 programs CD4+ T-cell activation and effector T-cell differentiation in systemic lupus erythematosus Stem Cells Transl Med.
This research paper investigated the role of Wilms tumor 1-associated protein (WTAP) in osteoporosis and the differentiation of bone marrow mesenchymal stem cells (BMMSCs). The study demonstrated that WTAP promoted osteogenic differentiation and inhibited adipogenic differentiation of BMMSCs through the miR-29b-3p/HDAC4 axis. WTAP-mediated m6A modification negatively regulated osteoclast differentiation, suggesting WTAP as a potential therapeutic target for osteoporosis treatment.
(Products Used: EpiQuik m6A RNA Methylation Quantification Kit (Colorimetric))

Lu S et. al. (April 2023). WTAP-Mediated m6A RNA Methylation Regulates the Differentiation of Bone Marrow Mesenchymal Stem Cells via the miR-29b-3p/HDAC4 Axis Stem Cells Transl Med.
The research investigated the role of m6A methyltransferase like 3 (METTL3) in CD4+ T-cell activation, differentiation, and systemic lupus erythematosus (SLE) pathogenesis. The study found that METTL3 was defective in CD4+ T-cells of SLE patients and that its inhibition promoted CD4+ T-cell activation and affected the differentiation of effector T-cells, suggesting that METTL3 could serve as a potential therapeutic target for SLE treatment.
(Products Used: EpiQuik m6A RNA Methylation Quantification Kit (Colorimetric))

Zhao Z et. al. (April 2023). The putative methyltransferase LaeA regulates mycelium growth and cellulase production in Myceliophthora thermophila Biotechnol Biofuels Bioprod. 16(1):58.
The paper investigates the role of the putative methyltransferase LaeA in regulating mycelium growth, sugar consumption, and cellulases expression in the thermophile fungus Myceliophthora thermophila. The authors found that deletion of LaeA significantly enhanced mycelium growth and glucose consumption and increased extracellular protein and endo-glucanase activity. LaeA also regulated multiple growth regulatory factors and was associated with modulating H3K9 methylation levels, suggesting it could be a target for improving fermentation properties of industrial fungal strains.
(Products Used: EpiQuik Global Histone H3K4 Methylation Assay Kit, EpiQuik Global Histone H3K9 Methylation Assay Kit, EpiQuik Global Histone H3K27 Methylation Assay Kit)