BackgroundSingle-stranded DNA-binding protein that eliminates secondary structure in long ssDNA formed on the lagging strand of the replication fork. Stimulates DNA polymerase activity and increases the efficiency of RNA primer synthesis by interacting with the DNA polymerase and the helicase/primase protein gp4. Disrupts loops, hairpins and other secondary structures present on ssDNA to reduce and eliminate pausing of viral DNA polymerase at specific sites during elongation.
DescriptionSSB Protein gp2.5 Polyclonal Antibody, Biotin Conjugated. Biotin. Raised in: Rabbit.
FormulationLiquid. 0.03% Proclin 300, 50% Glycerol, 0.01M PBS, PH 7.4.
SpecificityEnterobacteria phage T7
IsotypeIgG
Uniprot IDP03696
Purification>95%, Protein G purified
ImmunogenRecombinant Enterobacteria phage T7 Single-stranded DNA-binding protein gp2.5 protein (1-232AA)
StorageShipped at 4°C. Upon delivery aliquot and store at -20°C (short-term) or -80°C (long-term). Avoid repeated freeze.
Alternative NamesSSB protein, 2.5
ApplicationELISA; Recommended dilution: ELISA 1:500-1:1000