BackgroundPlays important roles in the production of 11-cis retinal and in visual pigment regeneration. The soluble form binds vitamin A (all-trans-retinol), making it available for LRAT processing to all-trans-retinyl ester. The membrane form, palmitoylated by LRAT, binds all-trans-retinyl esters, making them available for IMH (isomerohydrolase) processing to all-cis-retinol. The soluble form is regenerated by transferring its palmitoyl groups onto 11-cis-retinol, a reaction catalyzed by LRAT. The enzymatic activity is linearly dependent of the expression levels and membrane association.
DescriptionRPE65 Polyclonal Antibody. Unconjugated. Raised in: Rabbit.
Formulation0.03% Proclin 300. 50% Glycerol, 0.01M PBS, pH 7.4.
SpecificityHuman, Rat
IsotypeIgG
Uniprot IDQ16518
PurificationProtein G purified
ImmunogenRecombinant Human Retinoid isomerohydrolase protein (348-484AA)
StorageShipped at 4°C. Upon receipt, store at -20°C (short-term) or -80°C (long-term). Avoid repeated freeze.
Alternative NamesRetinoid isomerohydrolase, All-trans-retinyl-palmitate hydrolase, Retinal pigment epithelium-specific 65 kDa protein, Retinol isomerase, RPE65
ApplicationELISA, WB, IF; Recommended dilution: WB:1:500-1:5000, IF:1:200-1:500