Recombinant ACE2 Protein with His-Tag

Description
Recombinant Human ACE2 Protein with His tag is produced by HEK293 cells expression system. The target protein is expressed with sequence (Gln18-Ser740) of human ACE2 (Accession #Q9BYF1) fused with a 6×His tag at the N-terminus.

Bio-Activity
Measured by its binding ability in a functional ELISA. Immobilized recombinant Human ACE2-His at 2μg/mL (100 μL/well) can bind recombinant SARS-COV-2 S1- mFc Protein with a linear range of 0.49-9.31 ng/mL

Purity
> 90% by SDS-PAGE.

Endotoxin
< 0.1 EU/μg of the protein by LAL method.

Formulation
Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.C.

Species
Human

Expressed Host
HEK293 cells

Tag
N-6×His

Gene ID
59272

Uniprot ID
Q9BYF1

Storage
Store the lyophilized protein at -20°C to -80°C for long term. After reconstitution, the protein solution is stable at -20°C for 3 months, at 2-8°C for up to 1 week. 

Reconstitution
For lyophilized protein: Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water.

Alternative Names
ACE-2; ACEH; ACE2