BackgroundMammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein. Alternative splicing results in multiple transcript variants. Pseudogenes corresponding to this gene are found on chromosomes 6p and 12p. Read-through transcription also exists between this gene and the neighboring upstream lipoyltransferase 1 (LIPT1) gene.
DescriptionMRPL30 Polyclonal Antibody. Unconjugated. Raised in: Rabbit.
FormulationBuffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
SpecificityHuman, Rat
IsotypeIgG
Uniprot IDQ8TCC3
PurificationAffinity Purified
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-161 of human MRPL30 (NP_660213.1).
StorageShipped at 4°C. Upon receipt, store at -20°C. Avoid freeze / thaw cycles
Alternative NamesMRPL30; L28MT; L30MT; MRP-L28; MRP-L30; MRPL28; MRPL28M; RPML28; mitochondrial ribosomal protein L30
ApplicationWB, IHC; Recommended dilution: WB: 1:500-1:2000, IHC: 1:50-1:200