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EpiQuik™ Histone H3 Modification Multiplex Assay Kit (Colorimetric)
Simultaneously screen and measure 21 well-characterized H3 modifications in ~2.5 hours.
Streamlined Workflow and High-Throughput H3 Modification Profiling
Profile 21 histone H3 modifications in a single assay with this all-in-one, ELISA-based multiplex kit.
- Fast & efficient: Complete the entire procedure in ~2.5 hours.
- Convenience: Colorimetric readout—no radioactivity, electrophoresis, or chromatography; includes an assay control for each modification.
- High sensitivity: Detects as low as 0.5 ng/well per modification (20–500 ng/well input range).
- Reliable & normalized: Includes total histone H3 sets for accurate comparisons.
- Flexible & scalable: Strip-well format supports single runs or high-throughput screening.
Whether you’re running a few targets or all 21 at once, this kit delivers robust, reproducible results.
Compare Histone H3 Multiplex Assay vs DIY ELISA
| Feature | EpiQuik™ Histone H3 Modification Multiplex Assay Kit | DIY ELISA |
|---|---|---|
| Assay Format | ✅ Multiplexed (21 marks on one plate) | ❌ Single mark per plate (custom multiplexing required) |
| Protocol Time | ✅ Fast (2.5 hrs) | ❌ Slow (4 hrs – 2 days for multiplexing) |
| Setup / Optimization | ✅ Ready-to-use (no optimization needed) | ❌ Long setup (2 – 8 weeks for coating, blocking, titration, QC) |
| Starting Material | ✅ Flexible (histone extracts or purified H3) | ❌ Variable (depends on antibody; requires prep/verification) |
| Sample Requirement | ✅ Low (20 – 500 ng/well) | ❌ High (10 – 1000 ng/well; must optimize) |
| Assay Control | ✅ Included recombinant H3 protein | ❌ Must source/prepare your own controls |
| Quantification | ✅ Reliable (relative + absolute) | ❌ Variable (depends on setup and antibody) |
| Cost per Reaction | ✅ Affordable ($8 – $10) | ❌ Expensive (>$50*) |
| Peer-reviewed Citations | ✅ 50+ references | ❌ Not well cited |
*Assumes fixed cost of 21 antibodies and optimization labor.
Featured in the world’s leading scientific journals
Our Histone H3 Modification Multiplex Assay Kit are trusted by leading researchers and cited in top-tier publications with high impact factors.
Impact Factor: 69.5
Jaccard A et al. (Sep 2023). Reductive carboxylation epigenetically instructs T cell differentiation . Nature.
Impact Factor: 27.2
Chevalier C et al. (Jul 2024). Epithelial cells maintain memory of prior infection via H3 di-methylation . Nature Communications.
Impact Factor: 20.8
Kovatcheva M et al. (Nov 2023). Vitamin B12 is a limiting factor for induced cellular plasticity and tissue repair . Cell Stem Cell.
Impact Factor: 15.1
Ng KY et al. (Mar 2021). Chemotherapy-Enriched THBS2-Deficient Cancer Stem Cells Drive Hepatocarcinogenesis through Matrix Softness-Induced Histone H3 Modifications . Advanced Science.
Get ready for fast, accurate Histone Modification Quantification
View Product DetailsFrequently asked questions
How can I measure multiple histone H3 modifications in a single assay?
The EpiQuik™ H3 Modification Multiplex Assay uses capture antibodies in a 96-well microplate, enabling simultaneous detection of 21 H3 marks in ~2.5 hours with a simple colorimetric readout.
Which histone H3 modifications are covered?
H3K4me1/2/3, H3K9me1/2/3, H3K27me1/2/3, H3K36me1/2/3, H3K79me1/2/3, H3K9ac, H3K14ac, H3K18ac, H3K56ac, H3Ser10P, and H3Ser28P.
What sample types and inputs are required?
Purified histone extracts, acid-extracted nuclei, or sheared chromatin. Typical input: 50–200 ng total histone per well.
How long is the workflow?
Hands-on time is under 30 minutes; total incubation/color development is approximately 2.5 hours.
Is it suitable for epigenetic drug screening?
Yes—profiling 21 marks in parallel is ideal for HDAC/HMT/demethylase inhibitor screening and other perturbation studies.
