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EpiQuik™ Histone H3 Modification Multiplex Assay Kit (Colorimetric)
Simultaneously screen and measure 21 well-characterized H3 modifications in ~2.5 hours.
Streamlined Workflow and High-Throughput H3 Modification Profiling
Profile 21 histone H3 modifications in a single assay with this all-in-one, ELISA-based multiplex kit.
- Fast & efficient: Complete the entire procedure in ~2.5 hours.
- Convenience: Colorimetric readout—no radioactivity, electrophoresis, or chromatography; includes an assay control for each modification.
- High sensitivity: Detects as low as 0.5 ng/well per modification (20–500 ng/well input range).
- Reliable & normalized: Includes total histone H3 sets for accurate comparisons.
- Flexible & scalable: Strip-well format supports single runs or high-throughput screening.
Whether you’re running a few targets or all 21 at once, this kit delivers robust, reproducible results.
Compare Histone H3 Multiplex Assay vs DIY ELISA
| Feature | EpiQuik™ Histone H3 Modification Multiplex Assay Kit | DIY ELISA |
|---|---|---|
| Assay Format | ✅ Multiplexed (21 marks on one plate) | ❌ Single mark per plate (custom multiplexing required) |
| Protocol Time | ✅ Fast (2.5 hrs) | ❌ Slow (4 hrs – 2 days for multiplexing) |
| Setup / Optimization | ✅ Ready-to-use (no optimization needed) | ❌ Long setup (2 – 8 weeks for coating, blocking, titration, QC) |
| Starting Material | ✅ Flexible (histone extracts or purified H3) | ❌ Variable (depends on antibody; requires prep/verification) |
| Sample Requirement | ✅ Low (20 – 500 ng/well) | ❌ High (10 – 1000 ng/well; must optimize) |
| Assay Control | ✅ Included recombinant H3 protein | ❌ Must source/prepare your own controls |
| Quantification | ✅ Reliable (relative + absolute) | ❌ Variable (depends on setup and antibody) |
| Cost per Reaction | ✅ Affordable ($8 – $10) | ❌ Expensive (>$50*) |
| Peer-reviewed Citations | ✅ 50+ references | ❌ Not well cited |
*Assumes fixed cost of 21 antibodies and optimization labor.
DNA-Methylation Assay Sensitivity & Speed
Featured in the world’s leading scientific journals
Our Histone H3 Modification Multiplex Assay Kit are trusted by leading researchers and cited in top-tier publications with high impact factors.
Impact Factor: 69.5
Jaccard A et al. (Sep 2023). Reductive carboxylation epigenetically instructs T cell differentiation . Nature.
Impact Factor: 27.2
Chevalier C et al. (Jul 2024). Epithelial cells maintain memory of prior infection via H3 di-methylation . Nature Communications.
Impact Factor: 20.8
Kovatcheva M et al. (Nov 2023). Vitamin B12 is a limiting factor for induced cellular plasticity and tissue repair . Cell Stem Cell.
Impact Factor: 15.1
Ng KY et al. (Mar 2021). Chemotherapy-Enriched THBS2-Deficient Cancer Stem Cells Drive Hepatocarcinogenesis through Matrix Softness-Induced Histone H3 Modifications . Advanced Science.
Get ready for fast, accurate Histone Modification Quantification
View Product DetailsFrequently asked questions
How can I measure multiple histone H3 modifications in a single assay?
The EpiQuik™ H3 Modification Multiplex Assay uses capture antibodies in a 96-well microplate, enabling simultaneous detection of 21 H3 marks in ~2.5 hours with a simple colorimetric readout.
Which histone H3 modifications are covered?
H3K4me1/2/3, H3K9me1/2/3, H3K27me1/2/3, H3K36me1/2/3, H3K79me1/2/3, H3K9ac, H3K14ac, H3K18ac, H3K56ac, H3Ser10P, and H3Ser28P.
What sample types and inputs are required?
Purified histone extracts, acid-extracted nuclei, or sheared chromatin. Typical input: 50–200 ng total histone per well.
How long is the workflow?
Hands-on time is under 30 minutes; total incubation/color development is approximately 2.5 hours.
Is it suitable for epigenetic drug screening?
Yes—profiling 21 marks in parallel is ideal for HDAC/HMT/demethylase inhibitor screening and other perturbation studies.
