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DAPK2 Polyclonal Antibody

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Western blot
All lanes: DAPK2 Polyclonal Antibody at 6ug/ml+ Mouse brain tissue
Goat polyclonal to rabbit at 1/10000 dilution
Predicted band size: 43, 56 kDa
Observed band size: 43 kDa
Immunohistochemistry of paraffin-embedded human skeletal muscle tissue using DAPK2 Polyclonal Antibody at dilution 1:100
Immunohistochemistry of paraffin-embedded human breast cancer tissue using DAPK2 Polyclonal Antibody at dilution 1:100
Immunohistochemistry of paraffin-embedded human liver cancer tissue using DAPK2 Polyclonal Antibody at dilution 1:100
Immunofluorescent analysis of MCF-7 cells using DAPK2 Polyclonal Antibody at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L)
Application: ELISA, IF, IHC, WB
Areas of Research: Apoptosis & Cell Death, Signal Transduction
Clonality: Polyclonal
Conjugate: Unconjugated
Host: Rabbit
Isotype: IgG
Purification: >95%, Protein G Purified
Reactivity: Human, Mouse
Trial Size Available: Yes
100% Guarantee: 6 months
Catalog No.SizePriceQty
A50185-02020 µg $99.00 
A50185-05050 µg $256.00 
A50185-100100 µg $359.00 
Availability: Usually Ships Within 1 Week 
Product Overview

Background
Calcium/calmodulin-dependent serine/threonine kinase involved in multiple cellular signaling pathways that trigger cell survival, apoptosis, and autophagy. Regulates both type I apoptotic and type II autophagic cell deaths signal, depending on the cellular setting. The former is caspase-dependent, while the latter is caspase-independent and is characterized by the accumulation of autophagic vesicles. Acts as a mediator of anoikis and a suppressor of beta-catenin-dependent anchorage-independent growth of malignant epithelial cells. May play a role in granulocytic maturation. Isoform 2 is not regulated by calmodulin. It can phosphorylate MYL9. It can induce membrane blebbing and autophagic cell death.

Description
DAPK2 Polyclonal Antibody. Unconjugated. Raised in: Rabbit.

Formulation
Liquid. 0.03% Proclin 300, 50% Glycerol, 0.01M PBS, PH 7.4.

Specificity
Human, Mouse

Isotype
IgG

Uniprot ID
Q9UIK4

Purification
>95%, Protein G purified

Immunogen
Recombinant Human Death-associated protein kinase 2 protein (222-370AA)

Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C (short-term) or -80°C (long-term). Avoid repeated freeze.

Alternative Names
DAP kinase 2, DAP-kinase-related protein 1, DRP-1, DAPK2

Application
ELISA, WB, IHC, IF; Recommended dilution: WB:1:500-2000, IHC:1:20-1:200, IF:1:50-1:200

User Guide & MSDS

[User Guide]*
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