Chromatin immunoprecipitation has become one of the most widely used methods for studying DNA protein interactions. See the typical protocol steps and our cost-efficient, convenient assay kit solutions.
Interactions between DNA and proteins are of great biological significance as they govern numerous physiological processes, including: DNA replication and recombination; gene transcription; chromosome segregation; signal transduction; and epigenetic silencing.
Chromatin immunoprecipitation, or ChIP, is one of the most popular methods employed to study protein-DNA interactions. ChIP can be used to identify the specific genomic DNA region that binds a protein of interest (e.g., modified histone; transcription factor) in living cells. A typical ChIP protocol involves the following sequential steps:
In vivo cross-linking of cells
Chromatin extraction
DNA shearing
Protein-DNA immunoprecipitation with antibody against target protein
Cross-link reversal
DNA purification
Downstream analysis (PCR, NGS)
Most ChIP methods are considerably time-consuming, are labor-intensive, or have low throughput. To address these issues, EpiGentek has developed a series of fast and easy ChIP products that contain all the essential components required to carry out a successful ChIP experiment in a high-throughput, microplate format. These reliable and consistent kits are compatible with all DNA amplification-based approaches (e.g., ChIP-PCR; ChIP-seq) and include spin columns for post-ChIP DNA purification, cutting down on both time and labor. The entire procedure, from cell/tissue sample to ready-to-use purified DNA eluate, can be completed within 5-6 hours.
ChIP Assay Kit Comparison Guide:
For your convenience we supply an array of effective, convenient assay kit solutions to provide what you need to carry out successful ChIP experiments:
Each kit listed is a high throughput microplate-based assay ("Matrix ChIP") to help increase the speed and efficiency of your experiments.
Providing complete sets of optimized reagents needed to perform chromatin immunoprecipitation in a convenient microplate-based format, the kits below come ready-to-use.
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