This is part of our end-to-end viral RNA modification workflow, click the image for more details

Viral RNA Extraction: Robust Spin-Column & Magnetic Based Kits

With the increasing focus on viral infectious diseases in the wake of the current SARS-CoV-2 pandemic, we want to ensure researchers have the tools they need to study the RNA that drives the spread and function of these viruses. EpiGentek has been a trusted research kit provider for over 15 years. In that time we have come to understand what researchers are looking for in terms of speed, reproducibility and ease.

That’s why we are offering robust viral RNA extraction kits alongside our cell/tissue RNA extraction kits, for a variety of options for completing the viral RNA preparation your lab needs to move forward with your projects. Each of our kits is designed to extract high-quality resulting RNA that can be used for RT-PCR in addition to a score of other downstream applications. Our kits vary in the method of extraction as well as the type of throughput options (high/manual), so no matter what your lab requires, our kits can help you obtain ready-to-use purified viral RNA.

Our variety of magnetic bead and spin column kits isolate RNA from different sample sources (nasal/nasopharyngeal swabs, cells/tissues) and species (viral, mammalian). Detergents and chaotropic salt are used to lyse cells and inactivate RNases. A specialized high salt buffering system allows RNA bases to bind to the magnetic beads to the glass fiber matrix of the spin columns while contaminants are efficiently washed away. Pure nucleic acid is eluted with an aqueous buffer; phenol extraction or alcohol precipitation are not required.

Viral RNA Extraction Kits

For viral RNA extraction, the P-9107 spin column kit affords the most benefits in terms of ease-of-use, speed, and yield. The P-9109 magnetic bead kit provides the convenience of high throughput sample processing in a 96-well plate setup and is suitable for robot handling or automation setup, without the need for centrifugation. Alternatively, the P-9108 kit can be used for manual magnetic bead isolation of smaller sample sizes. Note that, depending on the desired downstream application, additional virus purification steps may be needed to achieve the required yield and purity. To help you plan your studies accordingly, we've provided an article detailing 10 methods in which this process can be done.

Kit Type	Kit Details
Fast Spin Column-Based Assay	Click image to see more details	EpiQuik Viral RNA Isolation Fast Kit For rapid extraction of total viral RNA from saliva and nasal/nasopharyngeal swabs via spin columns Fastest: delivers high-quality RNA in 10 minutes Consistent Yield: recover quantities of up to 5 µg of purified RNA Size: 50 Samples Catalog #: P-9107-50
Highest Throughput	Click image to see more details	EpiMag 96-Well Viral RNA Extraction Kit (High Throughput) For magnetic bead-based high throughput extraction of total viral RNA from saliva and nasopharyngeal swabs High Throughput: extract up to 96 samples simultaneously Automation Capabilities: suitable for robotic handling and automation setups Fast: procedure completes within 30 minutes Convenient: no need for centrifugation Size: 96 Samples Catalog #: P-9109-96
Magnetic Bead-Based Assay	Click image to see more details	EpiMag Viral RNA Isolation Kit (Magnetic Beads) For magnetic bead-based extraction of total viral RNA from saliva and nasopharyngeal swabs Rapid: delivers high-quality RNA in 25 minutes Versatile: allows small size (>30 bases) and large size (<200 kb) to be isolated Convenient: no need for centrifugation Size: 50 Samples Catalog #: P-9108-50

If you would like to order multiple RNA extraction kits, we also make it convenient by allowing you to purchase via a workflow. This way, you can get the assay kits you need and simultaneously view other related kits that may fit your study, including downstream applications like RNA methylation quantification.

Cell/Tissue RNA Extraction Kits

As an alternative to the kits presented above, we also offer kits targeted at isolating RNA derived from mammalian cells and tissue. If your study plans to use cells or tissues as your starting input material, the P-9105 and P-9106 kits provide the same high quality extraction of total RNA via column- and bead-based formats, respectively.

Kit Type	Kit Details
Spin Column-Based Assay	Click image to see more details	EpiQuik Total RNA Isolation Fast Kit Fast, simple, and cost-effective method for the isolation of total RNA from mammalian cells and tissues via spin columns Rapid Procedure: delivers high-quality total RNA in 25-40 minutes Consistent Yield: recover quantities of up to 10 µg of purified RNA Low Amount of Starting Material: starting materials can be up to 1 X106 mammalian cells, or 10 mg tissues Size: 50 Samples Catalog #: P-9105-50
Magnetic-Based Assay	Click image to see more details	EpiQuik Magbeads Quick RNA Isolation Kit Rapid & easy method for the isolation of total RNA from mammalian cells and tissues via magnetic beads Fast Procedure: delivers high-quality total RNA in 25-40 minutes Consistent Yield: recover quantities of up to 10 µg of purified RNA Low Amount of Starting Material: starting materials can be up to 1 X106 mammalian cells, or 10 mg tissues Size: 50 Samples Catalog #: P-9106-50

Also, be sure to see how you can add multiple RNA extraction products at once using our handy purchase via a workflow feature.

Other RNA Extraction Protocols

The above kits are designed to include all necessary reagents and materials to extract RNA from either viral samples or cells and tissue. There are, however, other viable methods for achieving RNA extraction if your lab has the materials abailable. Below we have included links to a few protocols that we that you can follow to isolate and purify RNA from different sources:

**Note: these are suggested protocols and should be adjusted by the user accordingly**

• Ultracentrifugal RNA Virus Purification
• RNA Virus Amplification
• Phenol/Chloroform RNA Extraction
• Concentrating RNA Viruses by PEG Precipitation

Have questions about which viral RNA extraction kit is right for your particular needs? Feel free to give us a call at (631) 755-0888 or send us a message through our contact form. One of our representatives will be happy to field any questions and get you the right kits for your current projects.

Studying COVID-19? See our new collection of SARS-CoV-2 Related Antibodies, Proteins & Assays.