The EpiMag™ Viral RNA Isolation Kit is a complete set of optimized buffers and reagents suitable for quick preparation of viral RNA from cell-free liquid specimens (excluding plasma and serum), specifically from saliva and nasal or nasopharyngeal swabs. The specialized buffering system allows RNA to bind to the magnetic beads while contaminants and impurities are efficiently washed away, and pure RNA is eluted. The RNA purified with the kit is suitable for a variety of routine applications, specifically for RT-PCR. This kit has the following advantages and features:
Total RNA was isolated from nasal swab samples with the EpiMag™ Viral RNA Isolation Kit and used for RT-PCR analysis. A: Nasopharyngeal swab sample was spiked with SARS-CoV-2-N positive control; B: Nasopharyngeal swab sample was not spiked with SARS-CoV-2-N positive control. PCR was processed with the use of primers and probes against SARS-CoV-2 N1/N2 and human RNase P gene (internal control).
Principle & ProcedureThe kit contains all the reagents required for successfully performing RNA isolation directly from cell-free samples (excluding plasma and serum). After lysis, binding, and wash, RNA is easily recovered in quantities of up to 5 µg using specially designed magnetic beads. Total RNA is then ready to be used for a variety of downstream applications.
Starting MaterialsThe amount of starting materials can be up to 400 µl of liquid volume, with the best volume of 200 µl. A total of 50 standard extractions (using 200 µl of the sample) can be performed with this kit.