SOD1 Recombinant Monoclonal Antibody [3E5]

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Overlay histogram showing Jurkat cells stained with SOD1 rMAb (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then incubated in 10% normal goat serum to block non-specific protein-protein interactions followedby the antibody (1µg/1*10⁶ cells) for 1 h at 4°C. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 30min at 4°C. Control antibody (green line) was Rabbit IgG (1µg/1*10⁶ cells) used under the same conditions. Acquisition of >10,000 events was performed.

Western Blot
Positive WB detected in: 293 whole cell lysate, Hela whole cell lysate, Jurkat whole cell lysate, MCF-7 whole cell lysate, K562 whole cell lysate, HepG2 whole cell lysate, U87 whole cell lysate
All lanes: SOD1 antibody at 1:1500
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 16 kDa
Observed band size: 18 kDa

Application:	ELISA, FC, WB
Areas of Research:	Protein Metabolism
Clonality:	Monoclonal
Conjugate:	Unconjugated
Host:	HEK293F Cell
Isotype:	Rabbit IgG
Purification:	Affinity Chromatography
Reactivity:	Human
Recombinant:	Yes
100% Guarantee:	6 months

Catalog No.	Size	Price	Qty
A74329-050	50µl	$299.00
A74329-100	100µl	$399.00

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Product Overview
Datasheet & SDS
Product FAQ

Product Overview

Background
Destroys radicals which are normally produced within the cells and which are toxic to biological systems.

Description
SOD1 Recombinant Monoclonal Antibody [3E5]. Unconjugated. Raised in: HEK293F Cell.

Formulation
Buffer: Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Specificity
Human

Isotype
Rabbit IgG

Uniprot ID
P00441

Purification
Affinity-chromatography

Immunogen
A synthesized peptide derived from human SOD1

Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Alternative Names
Superoxide dismutase [Cu-Zn] (EC 1.15.1.1) (Superoxide dismutase 1) (hSod1), SOD1

Application
ELISA, WB, FC; Recommended dilution: WB:1:500-1:5000, FC:1:20-1:200

Datasheet & SDS

Datasheet/User Guide
If the above file is not available online, you can request a copy by contacting us.

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SOD1 Recombinant Monoclonal Antibody [3E5]