DescriptionRecombinant SARS-COV-2 S1+S2 ECD(S-ECD) Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Val11-Gln1208) of SARS-COV-2 S1+S2 ECD(S-ECD) (Accession #YP_009724390.1) fused with a 6×His tag at the C-terminus. It is the wildtype sequence in pre-fusion state without any mutation.Bio-ActivityMeasured by its binding ability in a functional ELISA. Immobilized SARS-CoV-2 S1+S2 ECD(S-ECD) at 2μg/mL (100 μL/well) can bind recombinant Human ACE2 with a linear range of 0.15-3.72 ng/mL.
Purity> 95% by SDS-PAGE.
Endotoxin< 0.1 EU/μg of the protein by LAL method.
FormulationSupplied as a 0.22 μm filtered solution in PBS, pH 7.4.
SpeciesSARS-CoV-2
Expressed Host HEK293 cells
Tag6×His tag at the C-terminusGene ID 43740568
StorageThe product is shipped with ice or equivalent. Upon receipt, store it immediately at ≤ -70°C for. For optimal storage, aliquot into smaller quantities after centrifugation and store at the recommended temperature. Avoid repeated freeze/thaw cycles.
Alternative Namescov spike Protein; 2019-nCoV;ncov spike Protein; 2019-nCoV;coronavirus spike Protein; 2019-nCoV;S glycoprotein; COVID-19;S protein; Spike glycoprotein