Histone Methylation Quantification

Histone methylation is the modification of certain amino acids in a histone protein by the addition of methyl groups. Methylation and demethylation of histones turn genes on and off in DNA. Quantifying histone methylation is particularly useful for studying gene expression patterns.

---

	Histone H3 Modification Multiplex Assay Kit Cat #P-3100	Histone H4 Modification Multiplex Assay Kit Cat #P-3102
	Citations	Citations
Purpose	For ELISA-like quantitation of 21 different histone H3 modification patterns on the same plate	For ELISA-like quantitation of 10 different histone H4 modification patterns on the same plate
Key Points	Simultaneously screen and measure 21 different histone H3 modifications Detection threshold as low as 0.5 ng/well Useful for cost effectively identifying potential ChIP-qPCR or ChIP-seq targets	Simultaneously screen and measure 10 different histone H4 modifications Detection threshold as low as 0.5 ng/well Useful for cost effectively identifying potential ChIP-qPCR or ChIP-seq targets
Starting Material	Total Histone Extracts/ Purified H3 Proteins	Total Histone Extracts/ Purified H4 Proteins
Input Amount	20-500 ng	20-500 ng
Total Protocol Time	2.5 hours	2.5 hours
Reactions	96	96
Vessel Format	96-Well Plate	96-Well Plate

---