Secondary Antibodies

What are Secondary Antibodies

Secondary antibodies are crucial reagents used in many research applications, particularly in the field of immunology. They are typically used in conjunction with primary antibodies, which are specific to the target antigen of interest. The primary antibody binds to the antigen, while the secondary antibody binds to the primary antibody and provides a means to detect and quantify the antigen-antibody complex.

Applications of Secondary Antibodies

Secondary antibodies are used in a wide range of techniques such as Western blot, ELISA, Immunohistochemistry, Immunofluorescence, Flow Cytometry, etc. They are extremely important in these techniques because they allow the detection of low levels of antigens, and they also increase the specificity/sensitivity of the assay.

Conjugation of Secondary Antibodies

Secondary antibodies are commonly conjugated to enzymes such as horseradish peroxidase (HRP) or alkaline phosphatase (AP) to facilitate detection, or to fluorescent dyes for use in fluorescence-based techniques. The use of secondary antibodies also allows for multiplexing, where multiple different antigens can be detected in the same sample simultaneously.

What makes buying antibodies from EpiGentek different?

At EpiGentek, we supply a collection of over 20,000 individual high-quality antibodies for our customers to ensure that our research customers find exactly what they need for their studies. In addition, we offer a variety of other benefits to help our customers in terms of affordability, convenience, and reliability. See why working with EpiGentek for your antibodies is the right choice:

• No Shipping & Handling Costs: we’ve waived all shipping and handling fees on our antibodies and antibody panel packs when selecting 2-day shipping (trial sizes not applicable).
• 100% Application Guarantee: your purchased antibodies are covered for 6-months past the date of purchase.
• Multiple Conjugates: we carry biotin, FITC and HRP conjugates for nearly all targets.
• Trial Sizes Available: 10 & 20 µl options available for thousands of antibodies in our catalog.