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HAT & HDAC Assay Kits

Quickly and Easily Measure HAT or HDAC Levels

Histone acetyltransferases (HATs) and deacetylases (HDACs) are two classes of enzymes that catalyze reversible histone acetylation. Identifying and determining the distribution of HAT or HDAC activities can further our knowledge of cellular regulation and response. EpiGentek offers several ready-to-use kits that measure histone acetyltransferase or histone deacetylases levels quickly and safely.

Schematic procedure for the Epigenase™ HDAC Activity/Inhibition Direct Assay Kit (Cat# P-4034).

About Histone Acetylation and Deacetylation
The balance between protein acetylation and deacetylation regulates several physiological and pathological cellular processes, and the enzymes involved in the maintenance of this equilibrium -- histone acetyltransferases (HATs) and histone deacetylases (HDACs) -- have been widely studied. Presently, the evidence obtained in this field suggests that the dynamic acetylation equilibrium is primarily maintained through the physical and functional exchange between HAT and HDAC activities.

Kit Details

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EpiQuik HAT Activity/Inhibition Assay Kit
Measure histone acetyltransferase activity/ inhibition safely and quickly

Quick and easy 3-hour procedure
No need for radioactivity, extraction, and chromatography
Directly measure HAT activity/inhibition by quantifying the amount of acetylated histone substrate, avoiding the false inhibitory effect on HATs
Flexible strip-well microplate format for manual or high throughput analysis

Catalog #: P-4003

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Epigenase HDAC Activity/Inhibition Direct Assay Kits (Colorimetric and Fluorometric*)
Measure total activity/inhibition of HDAC enzymes in nuclear extracts or purified HDAC isoforms (HDACs 1-11)

Directly detects HDAC-converted deacetylated products rather than trypsin-based peptide cleavage
Uses cell/tissue extracts or purified HDAC enzymes for in vivo and in vitro detection
Detection from as low as 0.5 ng of purified HDAC enzyme
Includes a potent inhibitor of class I & II HDAC activity as the positive control
Flexible strip-well microplate format for manual or high throughput analysis

Catalog #s: P-4034 & P-4035*

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EpiQuik HDAC1 -7 Assay Kits
Measure the total amount of HDAC 1-11 proteins quantitatively through an ELISA-like method

No need for electrophoresis
Detection limit as low as 0.2 ng of HDAC protein
Flexible strip-well microplate format for manual or high throughput analysis

Catalog #s: See Cat#s

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HAT & HDAC Assay Kits

Quickly and Easily Measure HAT or HDAC Levels