This is part of our Histone Modification Workflow,
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HAT & HDAC Assay Kits

Quickly and Easily Measure HAT or HDAC Levels

Histone acetyltransferases (HATs) and deacetylases (HDACs) are two classes of enzymes that catalyze reversible histone acetylation. Identifying and determining the distribution of HAT or HDAC activities can further our knowledge of cellular regulation and response. EpiGentek offers several ready-to-use kits that measure histone acetyltransferase or histone deacetylases levels quickly and safely.

About Histone Acetylation and Deacetylation
The balance between protein acetylation and deacetylation regulates several physiological and pathological cellular processes, and the enzymes involved in the maintenance of this equilibrium -- histone acetyltransferases (HATs) and histone deacetylases (HDACs) -- have been widely studied. Presently, the evidence obtained in this field suggests that the dynamic acetylation equilibrium is primarily maintained through the physical and functional exchange between HAT and HDAC activities.

Kit Details
Click image to see more details	EpiQuik HAT Activity/Inhibition Assay Kit Measure histone acetyltransferase activity/ inhibition safely and quickly Quick and easy 3-hour procedure No need for radioactivity, extraction, and chromatography Directly measure HAT activity/inhibition by quantifying the amount of acetylated histone substrate, avoiding the false inhibitory effect on HATs Flexible strip-well microplate format for manual or high throughput analysis Catalog #: P-4003
Click image to see more details	Epigenase HDAC Activity/Inhibition Direct Assay Kits (Colorimetric and Fluorometric*) Measure total activity/inhibition of HDAC enzymes in nuclear extracts or purified HDAC isoforms (HDACs 1-11) Directly detects HDAC-converted deacetylated products rather than trypsin-based peptide cleavage Uses cell/tissue extracts or purified HDAC enzymes for in vivo and in vitro detection Detection from as low as 0.5 ng of purified HDAC enzyme Includes a potent inhibitor of class I & II HDAC activity as the positive control Flexible strip-well microplate format for manual or high throughput analysis Catalog #s: P-4034 & P-4035*
Click image to see more details	EpiQuik HDAC1 -7 Assay Kits Measure the total amount of HDAC 1-11 proteins quantitatively through an ELISA-like method No need for electrophoresis Detection limit as low as 0.2 ng of HDAC protein Flexible strip-well microplate format for manual or high throughput analysis Catalog #s: See Cat#s