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BNP Polyclonal Antibody

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Immunofluorescence analysis of L929 cells using BNP Polyclonal Antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
Western blot analysis of extracts of rat heart, using BNP Polyclonal Antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.

Exposure time: 30s.
Western blot analysis of extracts of mouse pancreas, using BNP Polyclonal Antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.

Exposure time: 30s.
Application: IF, WB
Areas of Research: Secreted Growth Factor Antibodies
Clonality: Polyclonal
Conjugate: Unconjugated
Host: Rabbit
Isotype: IgG
Purification: Affinity Purified
Reactivity: Mouse, Rat
Trial Size Available: A70586-020
100% Guarantee: 6 months
Catalog No.SizePriceQty
A70586-05050 µl $256.00 
A70586-100100 µl $359.00 
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Product Overview

Background
This gene is a member of the natriuretic peptide family and encodes a secreted protein which functions as a cardiac hormone. The protein undergoes two cleavage events, one within the cell and a second after secretion into the blood. The protein's biological actions include natriuresis, diuresis, vasorelaxation, inhibition of renin and aldosterone secretion, and a key role in cardiovascular homeostasis. A high concentration of this protein in the bloodstream is indicative of heart failure. The protein also acts as an antimicrobial peptide with antibacterial and antifungal activity. Mutations in this gene have been associated with postmenopausal osteoporosis.

Description
BNP Polyclonal Antibody. Unconjugated. Raised in: Rabbit.

Formulation
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Specificity
Mouse, Rat

Isotype
IgG

Uniprot ID
P16860

Purification
Affinity Purification

Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-134 of human BNP (NP_002512.1).

Storage
Shipped at 4°C. Upon receipt, store at -20°C. Avoid freeze / thaw cycles

Alternative Names
NPPB; BNP; natriuretic peptide B

Application
WB, IF; Recommended dilution: WB 1:500 - 1:2000, IF 1:50 - 1:200

User Guide & MSDS

[User Guide]*
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