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SpeedRun™ One-Hour ELISA

One-Hour ELISA Kits for Faster Target Quantification

SpeedRun™ One-Hour ELISA Kits are designed to shorten the path from sample to readout with a streamlined assay workflow for routine target quantification.

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SpeedRun One-Hour ELISA Kits

A faster ELISA format for a more efficient workday

The SpeedRun™ family is designed around one clear goal: reduce the time required to complete an ELISA while keeping the workflow easy to follow across the product line.

SpeedRun one-hour ELISA workflow

One-hour workflow

Plan the assay around a shorter completion window instead of a multi-hour ELISA schedule.

Streamlined SpeedRun ELISA workflow steps

Streamlined steps

A simplified sequence of assay steps is designed to reduce workflow interruptions and make timing easier to manage.

Familiar microplate ELISA workflow

Familiar ELISA format

Built around a familiar microplate ELISA format for straightforward integration into routine laboratory workflows.

Choose a SpeedRun™ One-Hour ELISA Kit

Explore the SpeedRun™ product family and select the assay that matches your research target.

5-mC DNA Quantitation

SpeedRun™ One-Hour 5-mC ELISA Kit (Colorimetric)

Input: DNA: 20 - 200 ng (100 ng optimal).

Cat# P-7100

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5-hmC DNA Quantitation

SpeedRun™ One-Hour 5-hmC ELISA Kit (Colorimetric)

Input: DNA: 50 - 200 ng (100 ng optimal).

Cat# P-7101

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m6A RNA Quantitation

SpeedRun™ One-Hour m6A RNA ELISA Kit (Colorimetric)

Input: RNA: 100 - 300 ng (200 ng optimal).

Cat# P-7102

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TET1 Protein Quantitation

SpeedRun™ One-Hour TET1 ELISA Kit (Colorimetric)

Input: Nuclear extract: 1 - 5 µg (2 - 4 µg optimal); Purified protein: 1 - 50 ng (5 - 10 ng optimal).

Cat# P-7103

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CRISPR/Cas9 Protein Quantitation

SpeedRun™ One-Hour CRISPR/Cas9 ELISA Kit (Colorimetric)

Input: Whole cell extract: 0.5 - 5 µg (1 - 2 µg optimal); Purified protein: 1 - 100 ng (10 - 50 ng optimal).

Cat# P-7104

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MBD2 Protein Quantitation

SpeedRun™ One-Hour MBD2 ELISA Kit (Colorimetric)

Input: Nuclear extract: 2 - 10 µg (2 - 4 µg optimal); Purified protein: 1 - 100 ng (20 - 50 ng optimal).

Cat# P-7105

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MBD1 Protein Quantitation

SpeedRun™ One-Hour MBD1 ELISA Kit (Colorimetric)

Input: Nuclear extract: 2 - 10 µg (2 - 4 µg optimal); Purified protein: 1 - 100 ng (20 - 50 ng optimal).

Cat# P-7106

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MBD3 Protein Quantitation

SpeedRun™ One-Hour MBD3 ELISA Kit (Colorimetric)

Input: Nuclear extract: 2 - 10 µg (2 - 4 µg optimal); Purified protein: 1 - 100 ng (20 - 50 ng optimal).

Cat# P-7107

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A shorter path from plate setup to readout

Our SpeedRun™ One-Hour ELISAs use an optimized assay workflow to significantly reduce total processing time while maintaining the sensitivity and reliability expected from EpigenTek ELISA technology.

Optimized buffer components enhance binding and reaction efficiency, enabling shorter incubation times. In addition, target capture and detection are combined into streamlined steps, reducing separate incubations and wash cycles required in conventional ELISA workflows.

SpeedRun™ workflow

Total assay time: about 1 hour
Step 1

Initial setup

Prepare standard curve and required solutions according to the kit user guide.

Step 2

Binding

Add controls and samples in High Binding Solution.

Step 3

Target capture

Incubate with Detection Complex Solution.

Step 4

Signal measurement

Add Developer Solution, and read the absorbance at 450 nm.

Representative quantitative performance

5-mC DNA Standard Curve

5-mC DNA standard curve for SpeedRun One-Hour 5-mC ELISA Kit P-7100

SpeedRun™ One-Hour 5-mC ELISA Kit (Cat# P-7100): representative standard curve generated with the 5-mC DNA standard. Actual results may vary.

MBD2 Protein Standard Curve

MBD2 protein standard curve for SpeedRun One-Hour MBD2 ELISA Kit P-7105

SpeedRun™ One-Hour MBD2 ELISA Kit (Cat# P-7105): representative standard curve generated with the MBD2 protein standard. Actual results may vary.

SpeedRun™ One-Hour ELISA FAQ

What is SpeedRun™ One-Hour ELISA?
SpeedRun™ is EpigenTek's one-hour ELISA product family, designed around a shortened assay timeline for target quantification. Target-specific technical details can be found on each individual kit page.
How long does a SpeedRun™ assay take?
The platform is designed around an approximately one-hour assay workflow. The one-hour timing is based on a representative single-point assay. Actual assay time may vary depending on the specific kit and experimental setup. Final step-by-step timing should follow the instructions supplied with the specific kit.
Which sample types can I use?
Supported sample types may differ by target, including DNA, RNA, and protein. Refer to the individual product page and kit user guide for recommended sample types for each SpeedRun™ kit.
What equipment is required?
SpeedRun™ kits use a microplate-based ELISA workflow. Confirm the required plate reader settings and any additional equipment in the individual kit user guide before running the assay.
Where can I find sensitivity, range, and performance data?
Target-specific performance data can be found on the individual product pages and in the kit user guides.
Why SpeedRun

Fit ELISA into the day more easily

Traditional ELISA workflows can take several hours and be difficult to schedule around other lab work. SpeedRun™ One-Hour ELISA Kits are designed to streamline the assay process, helping researchers move from setup to results in about one hour.

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Faster turnaround

Complete the assay within a shorter window so results can be reviewed sooner.

More predictable scheduling

A one-hour assay format can be easier to fit between other experiments, meetings, and routine lab tasks.

One Familiar Workflow Across the SpeedRun Line

A shared assay format reduces the need to learn a completely different workflow when moving from one SpeedRun™ target to another.

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