FARMINGDALE, NEW YORK – March 3, 2025 – Building on the success of its protein–DNA interaction technologies, EpigenTek is excited to announce the launch of its complete CUT&LUNCH platform, offering three powerful kits that enable researchers to go from intact cells to sequencing-ready DNA libraries faster and more efficiently than ever before.

EpiNext™ CUT&LUNCH Assay Kit (P-2035)

The foundational component of the CUT&LUNCH suite, this assay kit provides a rapid and high-specificity method for enriching target protein-DNA complexes.

• 3-step protocol completed in under 2 hours directly from cells—no ConA bead immobilization required.

• Low-input compatible, requiring as few as 2,000 cells.

• High specificity, with enzyme-directed cleavage at both ends of protein-bound chromatin regions, resulting in minimal background (~5%).

Learn more about P-2035:

Target protein/DNA enrichment using the EpiNext™ CUT&LUNCH Assay Kit. Histone/DNA complex was captured by the positive control antibody (H3K4me3) from 200,000 MCF-7 cells. Non-immune IgG was used as the negative control. The enriched DNA was purified and fluorescently quantified by Qubit or analyzed by qPCR with targeting to the GAPDH promoter for enrichment fold comparison.

EpiNext™ CUT&LUNCH Library Preparation Kit (P-1058)

This kit streamlines the conversion of CUT&LUNCH-enriched DNA into sequencing-ready libraries using a fast, single-tube format.

• Input range: 1–100 ng (optimal 10–50 ng).

• Complete library prep in <1 hour, including end-polish, adaptor ligation, size selection, and amplification.

• Ultra-HiFi enzyme minimizes bias while supporting both singleplex and multiplex indexing.

Learn more about P-1058:

Size distribution of library fragments. Enriched DNA from the CUT&LUNCH assay was used for DNA library preparation by the EpiNext™ CUT&LUNCH Library Preparation Kit. The peak reflects the insert size of DNA fragments (170-180 bps).

EpiNext™ CUT&LUNCH-Seq Kit (P-2033)

An all-in-one solution that integrates the CUT&LUNCH Assay and Library Prep into a single streamlined workflow—ideal for labs seeking convenience and speed.

• Start-to-finish in <3 hours, from cells to sequencing-ready DNA.

• Requires as few as 2,000 cells.

• Delivers high signal-to-noise and ENCODE-comparable data.

• Compatible with standard ChIP-seq data analysis pipelines.

Learn more about P-2033:

CUT&LUNCH histone marker peak profiles are consistent with ENCODE ChIP-seq profiles. Marker: H3K9me3. CUT&LUNCH: Peak scores are plotted in the bar graph for 0.1 million MCF-7 cells. ENCODE ChIP-Seq: Peak scores are plotted in the bar graph for MCF-7 cells (5-10 million).

CUT&LUNCH: A New Standard for Fast, Focused Chromatin Profiling

EpigenTek’s CUT&LUNCH platform delivers unprecedented speed and precision in mapping histone and transcription factor binding sites in chromatin:

• No ConA beads.
• No nuclei isolation.
• Minimal hands-on time.
• Low background, high enrichment.
• Low cell input requirements.
• Compatibility with any histone or strong-binding transcription factor.

Whether researchers prefer to run their own library prep or use an all-in-one system, the CUT&LUNCH family offers flexibility, scalability, and unmatched ease-of-use.

CUT&LUNCH histone marker peak profiles are consistent with ENCODE ChIP-seq profiles. Peak scores are plotted in the bar graph for 100,000 MCF-7 cells using the CUT&LUNCH assay kit, followed by NGS for histone H3K4me3. CUT&LUNCH shows lower background noise and narrower peaks.

About EpigenTek

EpigenTek is a New York-based biotechnology company that focuses on developing and providing innovative technologies, services, and products as a complete and systematic solution for epigenetics-related research and drug discovery.

For more information, technical resources, or to request a quote, please visit www.epigentek.com.