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DNA Methylation Quantification

DNA methylation plays an important role in normal organismal development and in cellular differentiation in higher organisms. Gene expression, as well as the development of nearly all types of cancer, are also tied to DNA methylation. For example, global decrease in 5-methylcytosine content (DNA hypomethylation) is likely caused by methyl-deficiency due to a variety of environmental influences, and has been proposed as a molecular marker in multiple biological processes such as cancer. Global quantification of DNA methylation is crucial for understanding the roles that gene expression and silencing play in the development of cancer and other diseases.

 

MethylFlash Methylated DNA Quantification Kit (Colorimetric)Cat #P-1034

MethylFlash Global DNA Methylation (5-mC) ELISA Easy Kit (Colorimetric)Cat #P-1030

MethylFlash Global DNA Hydroxymethylation (5-hmC) ELISA Easy Kit (Colorimetric)Cat #P-1032

MethylFlash 5-Formylcytosine (5-fC) DNA Quantification Kit (Colorimetric)Cat #P-1041

  More DetailsMore DetailsMore DetailsMore Details
PurposeColorimetrically measure global 5mC in DNAColorimetrically measure global 5mC in DNAColorimetrically measure global 5hmC in DNAColorimetrically measure global 5fC in DNA
Key Points
  • Highly cited method in major journals
  • Comparable results to HPLC-MS
  • Reduced cross-variation and improved accuracy
  • Fast and convenient "one-step" assay reaction
  • Robust 4th-generation MethylFlash technology applied to 5hmC studies
  • Robust MethylFlash technology applied to 5fC studies
Starting MaterialDNADNADNADNA
Input Range50-200 ng20-200 ng50-200 ng 100-300 ng
Optimal Input Amount100 ng100 ng100 ng300 ng
Total Time4 hours2 hours2 hours4 hours
Detection Threshold200 pg of meDNA50 pg of meDNA10 pg of hMeDNA1 pg of fC DNA
Type of Methylation Detected5-methylcytosine5-methylcytosine5-hydroxymethylcytosine5-formylcytosine
Specificity>1000:1 meDNA:unMeDNA>1000:1 meDNA:unMeDNA>1000:1 hMeDNA:unMeDNA>1000:1 fC DNA:unMeDNA
FormatELISA-like Microplate-basedELISA-like Microplate-basedELISA-like Microplate-basedELISA-like Microplate-based
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