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MethylFlash™ Global DNA Methylation (5-mC) ELISA Easy Kit
A fast, accurate, and reliable solution for quantifying global DNA methylation—trusted by researchers worldwide and backed by hundreds of citations.
Easy 3-Step Procedure with Gold-Standard Results
This ELISA-style kit streamlines 5-mC analysis with a quick, user-friendly protocol and robust performance across sample types.
- Fast & simple: Results in under 2 hours—no DNA denaturation or blocking.
- High sensitivity & specificity: Detects as low as 0.05% 5-mC with no cross-reactivity.
- Reliable & accurate: Optimized controls; results comparable to HPLC-MS.
- Flexible & universal: Strip-well format; compatible with ssDNA/dsDNA from any species.
- Proven & trusted: Highly cited in 300+ peer-reviewed studies.
Whether you’re running a few samples or scaling up, this kit delivers consistent, dependable results with unmatched convenience.
Compare Global 5-mC Quantification Kits
| Feature | EpigenTek 5-mC Kits | Competitor Z | Competitor A | Competitor C |
|---|---|---|---|---|
| Protocol Time | ✅ Fast (~2 hrs) | ❌ ~4 hrs | ❌ ~8 hrs | ❌ >4 hrs + overnight |
| Hands-on Prep | ✅ No denaturation/blocking | ❌ Denaturation + block | ❌ MseI digest + human probe | ❌ Digest → nucleosides + phosphatase |
| Compatible Species | ✅ Universal | ❌ Limited | ❌ Human only | ❌ Species-matched curve |
| Minimum DNA Input | ✅ 5 ng | ❌ 10 ng | ❌ ≥10 ng digested | ❌ ≥2 μg digested |
| Sensitivity | ✅ 0.05% 5-mC | ❌ ≥0.5% | ❌ ≥0.5% | ❌ ≥0.5% |
| Quantification | ✅ Relative + absolute | ❌ Relative only | ❌ Relative only | ❌ Relative only |
| Fluorometric Version | ✅ Yes (cat # P-1035) | ❌ No | ❌ No | ❌ No |
DNA-Methylation Assay Sensitivity & Speed
Featured In The World’s Leading Scientific Journals
Our DNA Methylation (5-mC) ELISA Kit is trusted by leading researchers and highly cited in top-tier publications.
Get Ready For Fast, Accurate Auantification Of Global DNA Methylation
View Product DetailsFrequently asked questions
What is the quickest way to quantify global DNA methylation (5-mC)?
MethylFlash™ Global DNA Methylation (5-mC) ELISA Easy Kit delivers results in under 2 hours with a simple 3-step workflow—no DNA denaturation or plate blocking required.
How sensitive is the assay?
It detects as low as 0.05% 5-mC using the recommended protocol and standard curve.
How much DNA do I need per well?
The kit accepts 5–200 ng DNA per well; ~100 ng is optimal for most samples.
Which DNA types and species are compatible?
Works with purified ssDNA or dsDNA from any species (e.g., human, mouse, plant, microbial).
Does the kit report absolute %5-mC or only relative changes?
You can report absolute %5-mC using the provided standards, or analyze relative differences across groups.
What plate format and reader settings are required?
96-well strip-well microplate; read on a standard microplate reader at ~450 nm.
What controls are included and how should I run replicates?
The kit includes standards/controls to build a calibration curve. We recommend duplicates for standards and samples; use triplicates when working near the LOD.
Does the assay cross-react with other cytosine modifications?
It is designed for high specificity to 5-mC with no cross-reactivity in the intended use.
Any quick troubleshooting tips?
- Low signal: confirm DNA amount/purity, incubation times, reagent prep; avoid under-washing.
- High background: reduce DNA input, increase wash stringency, prevent well drying.
- High CV: mix thoroughly, use consistent pipetting, run duplicates/triplicates.
What is the typical throughput per plate?
Up to 96 wells. Actual sample count depends on standards/controls and your replicate strategy.
Can I use fragmented or low-yield DNA?
Yes—fragmented or low-input DNA performs well within the 5–200 ng range. For scarce samples, use duplicates and ensure consistent handling.
How do results compare with HPLC-MS?
Global 5-mC levels measured with this kit show a strong correlation with HPLC-MS, using a faster and more accessible workflow.
