BackgroundActivates EIF2AK2/PKR in the absence of double-stranded RNA (dsRNA), leading to phosphorylation of EIF2S1/EFI2-alpha and inhibition of translation and induction of apoptosis. Required for siRNA production by DICER1 and for subsequent siRNA-mediated post-transcriptional gene silencing. Does not seem to be required for processing of pre-miRNA to miRNA by DICER1. Promotes UBC9-p53/TP53 association and sumoylation and phosphorylation of p53/TP53 at 'Lys-386' at 'Ser-392' respectively and enhances its activity in a EIF2AK2/PKR-dependent manner.
DescriptionPRKRA Polyclonal Antibody. Unconjugated. Raised in: Rabbit.
Formulation0.03% Proclin 300, 50% Glycerol, 0.01M PBS, PH 7.4
SpecificityHuman
IsotypeIgG
Uniprot IDO75569
PurificationProtein G purified
ImmunogenRecombinant Human Interferon-inducible double-stranded RNA-dependent protein kinase activator A protein (1-313AA)
StorageShipped at 4°C. Upon delivery aliquot and store at -20°C (short-term) or -80°C (long-term). Avoid repeated freeze.
Alternative NamesInterferon-inducible double-stranded RNA-dependent protein kinase activator A, PRKRA, PACT, RAX, HSD-14, HSD14, PKR-associated protein X, PKR-associating protein X, Protein activator of the interferon-induced protein kinase, Protein kinase, interferon-inducible double-stranded RNA-dependent activator
ApplicationELISA, IHC, IF; Recommended dilution: IHC:1:20-1:200, IF:1:50-1:500