FARMINGDALE, NY – February 2025 – EpigenTek continues to revolutionize epigenetics research with tools that combine speed, precision, and ease-of-use, helping scientists move from samples to insights faster than ever.  We are thrilled to introduce our ISAC (In Situ Accessible Chromatin) platform, delivering a streamlined and unbiased solution for chromatin accessibility analysis.  The platform includes three complementary kits that preserve chromatin integrity, minimize bias, and accelerate workflows.

EpiNext™ In Situ Accessible Chromatin (ISAC) Preparation Kit (P-1072)

• Purpose: Prepares in situ accessible chromatin from intact cells for PCR or NGS.

• Workflow: Complete in just 30 minutes, outperforming DNase/MNase/Tn5 methods (2-3 hours).

• Advantages:

• Preserves native chromatin structure with minimal nuclear perturbation.

• Requires low input and works on fresh/frozen samples.

• Sequence-independent cleavage avoids GC- or AT-bias typical of transposases.

Learn more about P-1072:

qPCR data of DNA purified from in situ accessible chromatin prepared with the EpiNext™ ISAC Preparation Kit, using 30,000 MCF-7 cells as input. Pink (HBB) and green (GAPDH) lines: Enzyme-treated; Blue (HBB) and Brown (GAPDH) lines: Untreated.

EpiNext™ ISAC Library Preparation Kit (P-1057)

• Purpose: Converts ISAC-cleaved DNA into Illumina-compatible libraries.

• Input Range: As low as 1 ng of DNA (10–50 ng recommended).

• Workflow: Single-tube format completes end-polish, adaptor ligation, cleanup, size selection, and amplification in ~1 hour, with no intermediate purifications.

• Advantages:

• Supports singleplex and multiplex barcoding.
  
  
• Ultra-HiFi amplification ensures low-bias, high-yield libraries with reproducible fragment sizes.

Learn more about P-1057:

EpiNext™ In Situ Accessible Chromatin Sequencing (ISAC-seq) Kit (P-1073)

• Purpose: All-in-one solution combining ISAC preparation and library construction for genome-wide accessibility profiling.

• Workflow: Full process—cells to sequencing-ready library—in only 1 hour and 35 minutes, dramatically faster than traditional ATAC-seq (2-10 hours).

• Advantages:

• Preserves chromatin integrity in situ without transposase.

• Works on small input amounts, including frozen samples.

• Offers broader site coverage and reduced bias versus ATAC-seq, DNase-seq, or MNase-seq.

• Incorporates all reagents for cleavage and sequencing prep in one kit.

Learn more about P-1073:

Representative fragment size data of DNA library prepared with the EpiNext™ ISAC seq Kit, using 30,000 MCF-7 cells as input.

Why ISAC Matters

EpigenTek’s ISAC workflow provides researchers with:

• Speed — Chromatin accessibility ready in just 30 minutes, full sequencing prep under 2 hours.

• Integrity — Maintains in situ chromatin structure with minimal manipulation.

• Unbiased profiling — Sequence-independent cleavage captures open chromatin broadly and sensitively.

• Low input friendly — Effective with small sample inputs, including frozen tissues.

• Ease-of-use — Modular kits offer flexibility, and the ISAC-seq kit provides a full solution.

• Broad utility — Applies to gene-specific PCR analysis or genome-wide NGS profiling.

About EpigenTek

EpigenTek is a New York-based biotechnology company that focuses on developing and providing innovative technologies, services, and products as a complete and systematic solution for epigenetics-related research and drug discovery.

For more information, technical resources, or to request a quote, please visit www.epigentek.com.