m6A Demethylase & Methylase Activity Kits

Get a clear view of the m6A activity in your samples using EpigenTek's convenient and simple ELISA-like assays. Each of our kits provides everything needed to measure m6A methylase or demethylase activity or inhibition levels from nuclear extracts or purified enzymes.

Measuring m6A Methylase Activity with EpigenTek's Assay Kit

EpigenTek’s Epigenase m6A Methylase Activity/Inhibition Assay Kit is designed for measuring the activity/inhibition of total m6A methylases (methyltransferases) using nuclear extracts or purified m6A methylases like METTL3/METTL14 from a broad range of species such as mammalian, plant, fungal, and bacterial, in a variety of forms including, but not limited to, cultured cells and fresh and frozen tissues.

With a straightforward protocol and accurate signal detection, EpigenTek's Epigenase m6A Methylase Activity/Inhibition Assay Kit is a valuable tool for researchers looking to investigate m6A methyltransferase levels, especially in regard to understanding how diseases like cancer progress over time. In a study published in the Journal of Experimental & Clinical Cancer Research, researchers examined m6A and m6A methyltransferase complex (METTL3) in both colorectal cancer (CRC) tissue and precancerous lesions to assess survival rates for patients. Using the Epigenase m6A Methylase Activity/Inhibition Assay Kit ( Cat.#P-9019) to assess methylase activity from the samples' nuclear extracts (Cat. #OP-0002), the team found significantly higher methylase activity in CRC compared to adenoma, with similar results observed for METTL3. These higher levels were also associated with poor prognosis. (Read the full study online here.*)

*Pan J et al. (January 2022). METTL3 promotes colorectal carcinoma progression by regulating the m6A-CRB3-Hippo axis. J Exp Clin Cancer Res. 41(1):19.

Featured Kits

Epigenase m6A Demethylase Activity/Inhibition Assay Kit (Colorimetric)

For measurement of total activity/inhibition of m6A demethylase from nuclear extracts or purified enzymes

• Fast: The entire procedure can be finished within 5 hours.
• High Sensitivity: Detection limit can be as low as 2 µg of nuclear extracts or 50 ng of purified enzymes.
• Robust: Innovative kit composition enables background signals to be extremely low, making the assay simple, accurate, reliable, and consistent.
• Quantitative: Includes assay standard to quantify the specific activity of m6A demethylase.
• Convenient: Both cell/tissue nuclear extracts and purified proteins can be used, allowing detection of inhibitory effects of m6A demethylase inhibitor in vivo and in vitro.
• Flexible: 96 strip-well microplate format makes the assay available for manual or high-throughput analysis.

Catalog #: P-9013

Epigenase m6A Methylase Activity/Inhibition Assay Kit (Colorimetric)

For measurement of total activity/inhibition of m6A methylases from nuclear extracts or purified enzymes

• Fast: The entire procedure can be finished within 4 hours.
• High Sensitivity: Detection limit can be as low as 2 µg of nuclear extracts or 50 ng of purified enzymes.
• Robust: Innovative kit composition enables background signals to be extremely low, making the assay simple, accurate, reliable, and consistent.
• Quantitative: Includes assay standard to quantify the specific activity of m6A methylase.
• Convenient: Both cell/tissue nuclear extracts and purified proteins can be used, allowing detection of inhibitory effects of m6A methylase inhibitor in vivo and in vitro.
• Flexible: 96 strip-well microplate format makes the assay available for manual or high-throughput analysis.

Catalog #: P-9019

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