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KIF22 Polyclonal Antibody

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IHC image of KIF22 Polyclonal Antibody diluted at 1:400 and staining in paraffin-embedded human kidney tissue performed on a Leica Bond™ system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Immunofluorescence staining of NIH/3T3 cells with KIF22 Polyclonal Antibody at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).
Application: ELISA, IF, IHC
Areas of Research: Cell Cycle
Clonality: Polyclonal
Conjugate: Unconjugated
Host: Rabbit
Isotype: IgG
Purification: Protein G Purified
Reactivity: Human
Trial Size Available: Yes
100% Guarantee: 6 months
Catalog No.SizePriceQty
A68859-02020 µg $99.00 
A68859-05050 µg $249.00 
A68859-100100 µg $399.00 
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Product Overview

Background
Kinesin family member that is involved in spindle formation and the movements of chromosomes during mitosis and meiosis. Binds to microtubules and to DNA. Plays a role in congression of laterally attached chromosomes in NDC80-depleted cells.

Description
KIF22 Polyclonal Antibody. Unconjugated. Raised in: Rabbit.

Formulation
Liquid. 0.03% Proclin 300, 50% Glycerol, 0.01M PBS, pH 7.4.

Specificity
Human

Isotype
IgG

Uniprot ID
Q14807

Purification
>95%, Protein G purified

Immunogen
Recombinant Human Kinesin-like protein KIF22 protein (34-169AA)

Storage
Shipped at 4°C. Upon receipt, store at -20°C (short-term) or -80°C (long-term). Avoid repeated freeze.

Alternative Names
Kinesin-like protein KIF22, Kinesin-like DNA-binding protein, Kinesin-like protein 4, KIF22, KID, KNSL4

Application
ELISA, IHC, IF; Recommended dilution: IHC: 1:200-1:500, IF: 1:50-1:200

User Guide & MSDS

[User Guide]*
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