ISAC-seq (In Situ Accessible Chromatin Sequencing) is a novel method developed by EpigenTek to greatly improve upon and address the issues of ATAC-seq, including:

• Rapid Protocol: Complete your assay in in 1 hour and 40 min.
• No Transposase Bias: No sequence-specific cleavage, minimizing fragmentation and GC/AT content biases.
• More Accessible Sites Covered: Sequence-independent binding allows for higher accessible chromatin site coverage.
• Cost-effective: Lower price per reaction compared to traditional chromatin accessibility assays.
• No Special Software:  Use existing, time-tested ChIP-seq bioinformatics pipelines for NGS data analysis (no special ATAC-seq settings are needed due to no transposase bias).