BackgroundComponent of the LUBAC complex which conjugates linear polyubiquitin chains in a head-to-tail manner to substrates and plays a key role in NF-kappa-B activation and regulation of inflammation. LUBAC conjugates linear polyubiquitin to IKBKG and RIPK1 and is involved in activation of the canonical NF-kappa-B and the JNK signaling pathways. Linear ubiquitination mediated by the LUBAC complex interferes with TNF-induced cell death and thereby prevents inflammation. LUBAC is proposed to be recruited to the TNF-R1 signaling complex (TNF-RSC) following polyubiquitination of TNF-RSC components by BIRC2 and/or BIRC3 and to conjugate linear polyubiquitin to IKBKG and possibly other components contributing to the stability of the complex. Together with FAM105B/otulin, the LUBAC complex regulates the canonical Wnt signaling during angiogenesis.
DescriptionSHARPIN Polyclonal Antibody, HRP Conjugated. HRP. Raised in: Rabbit.
FormulationLiquid. 0.03% Proclin 300, 50% Glycerol, 0.01M PBS, pH 7.4.
SpecificityHuman
IsotypeIgG
Uniprot IDQ9H0F6
Purification>95%, Protein G purified
ImmunogenRecombinant Human Sharpin protein (82-171AA)
StorageShipped at 4°C. Upon receipt, store at -20°C (short-term) or -80°C (long-term). Avoid repeated freeze.
Alternative NamesSharpin, Shank-associated RH domain-interacting protein, Shank-interacting protein-like 1, hSIPL1, SHARPIN, SIPL1, PSEC0216
ApplicationELISA; Recommended dilution: ELISA 1:500-1:1000